SpringerProtocols: Abstract: Isolation of Recombinant CardiacTroponin C

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Isolation of Recombinant CardiacTroponin C

By: John A. Putkey², Wen Liu²

Abstract

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Troponin C (TnC) is a founding member of the EF-hand family of Ca²⁺ binding proteins. It functions as the receptor for Ca²⁺on the thin filament of striated muscle. Association of Ca²⁺, with the regulatory Ca²⁺binding sites in TnC, induces muscle contraction. Subsequent release of Ca²⁺allows the muscle to relax. There are two forms of TnC; one is found in fast skeletal muscle (sTnC), whereas the other is found in cardiac and slow skeletal muscle (cTnC).

Affiliation(s): (2) Department of Biochemistry and Molecular Biology, University of Texas Medical Center, Houston, TX

Book Title: Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies

Series: Methods in Molecular Biology | Volume: 172 | Pub. Date: Jan-02-2002 | Page Range: 151-160 | DOI: 10.1385/1-59259-183-3:151

Subject: Protein Science

References

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