SpringerProtocols: Abstract: Methylation-Sensitive Restriction Fingerprinting

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Methylation-Sensitive Restriction Fingerprinting

By: Catherine S. Davies³

Abstract

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Methylation of cytosine residues is an almost ubiquitous finding of higher organisms (1). The majority of this methylation occurs at the dinucleotide CpG (where p denotes a phosphate group) (2). CpG sites are distributed throughout the genome with clusters of the sequence being found in the 5′ promoter region of housekeeping genes, in groups known as CpG islands. These short stretches of DNA have a have a C and G base composition, which in mammals and avians is estimated to be 10 times higher than in bulk DNA (3)

Affiliation(s): (3) Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, UK

Book Title: DNA Methylation Protocols

Series: Methods in Molecular Biology | Volume: 200 | Pub. Date: Mar-01-2002 | Page Range: 43-52 | DOI: 10.1385/1-59259-182-5:043

Subject: Genetics/Genomics

References

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