SpringerProtocols: Abstract: 12. Cloning PCR Products: An Overview

12. Cloning PCR Products: An Overview

By: Baotai Guo², Yuping Bi³

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A successful cloning of polymerase chain reaction (PCR)-derived DNA fragment is a key step for further analysis of the amplified DNAs, though it is often a difficult task. Many cloning methods have been established; various commercial cloning kits are also available. These methods can be separated into two groups: ligation-dependent cloning and ligation-independent cloning. The former is used more widely than the latter. According to DNA ends, the existing ligation-dependent cloning methods for PCR products can be further divided into three types: blunt-end cloning, sticky-end cloning, and T-A cloning.

Affiliation(s): (2) Laiyang Agricultural College, Institute of Plant Biotechnology, Shandong, China
(3) Shangdong Academy of Agricultural Sciences, Institute of Plant Biotechnology, Jinan, China

Book Title: PCR Cloning Protocols

Series: Methods in Molecular Biology | Volume: 192 | Pub. Date: Apr-01-2002 | Page Range: 111-119 | DOI: 10.1385/1-59259-177-9:111

Subject: Genetics/Genomics

References

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