SpringerProtocols: Abstract: 153. Phage Display: Biopanning on Purified Proteins and Proteins Expressed in Whole Cell Membranes

153. Phage Display: Biopanning on Purified Proteins and Proteins Expressed in Whole Cell Membranes

By: George K. Ehrlich², Wolfgang Berthold³, Pascal Bailon⁴

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Phage display technology (1) is rapidly evolving as a biomolecular tool with applications in the discovery of ligands for affinity chromatography (2-10) and drugs (11-17), in the study of protein-protein interactions (18-21), and in epitope mapping (22-26), among others. This technology relies on the utilization of phage display libraries (see Notes 1 and 2) in a screening process known as biopanning (27)

Affiliation(s): (2) Department of Pharmaceutical and Analytical R & D, Hoffman-LaRoche Inc., Nutley, NJ
(3) Division of Biopharmaceutical Sciences, IDEC Pharmaceuticals Corp., San Diego, CA
(4) Department of Pharmaceutical and Analytical R & D, Hoffmann-LaRoche Inc., Nutley, NJ

Book Title: The Protein Protocols Handbook

Series: No Series | Pub. Date: Feb-15-2002 | Page Range: 1059-1072 | DOI: 10.1385/1-59259-169-8:1059

Subject: Protein Science

References

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