SpringerProtocols: Abstract: Murine Intestinal Dendritic Cell Isolation

Murine Intestinal Dendritic Cell Isolation

By: Paul Pavli⁴

Abstract

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This chapter describes techniques for the isolation of dendritic cell-enriched single-cell suspensions from the lamina propria (LP) of both small and large intestine and from Peyer’s patches (PP). This technique can also be used to obtain intestinal macrophage-enriched populations. The characterization of an assay system, the allogeneic mixed leukocyte reaction (MLR), is also described.

Affiliation(s): (4) Gastroenterology, The Canberra Hospital, Australia

Book Title: Dendritic Cell Protocols

Series: Methods in Molecular Medicine | Volume: 64 | Pub. Date: Aug-01-2001 | Page Range: 63-72 | DOI: 10.1385/1-59259-150-7:63

Subject: Microbiology

References

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