SpringerProtocols: Abstract: Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells

Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells

By: Franz Koch⁴, Eckhart Kämpgen⁵, Gerold Schuler⁶, Nikolaus Romani⁴

Abstract

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Langerhans cells are the epidermal variant of the dendritic cell system (1-5). They were-unknowingly, though-the first dendritic cell to be described: In 1868 Paul Langerhans published his observations of a dendritically shaped cell in the human epidermis (6). Until the early 1990s (i.e., the advent of methods for mass production of dendritic cells from blood or bone marrow) Langerhans cells served as a prototype dendritic cell. The basis of our current knowledge on the maturation of dendritic cells stems from experiments with epidermal Langerhans cells (7-9).

Affiliation(s): (4) Department of Dematology, University of Innsbruck, Austria
(5) Department of Dermatology, University of Würzburg, Germany
(6) Deparment of Dermatology, University of Erlangen, Germany

Book Title: Dendritic Cell Protocols

Series: Methods in Molecular Medicine | Volume: 64 | Pub. Date: Aug-01-2001 | Page Range: 43-62 | DOI: 10.1385/1-59259-150-7:43

Subject: Microbiology

References

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