SpringerProtocols: Abstract: Restriction Fragment Length Polymorphism Typing of Mycobacteria

Restriction Fragment Length Polymorphism Typing of Mycobacteria

By: Dick van Soolingen³, Petra E. W. de Haas⁴, Kristin Kremer⁴

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In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS6110 RFLP typing of Mycobacterium tuberculosis complex isolates (1) and IS1245 RFLP typing of Mycobacterium avium strains (2). This chapter describes the technical details of these standardized methods regarding the isolation of DNA, restriction enzymes, electrophoresis conditions, internal- and external-size markers, Southern blotting, and several probes used for hybridization. Furthermore, RFLP typing of isolates of some other mycobacterial species is described.

Affiliation(s): (3) Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (Lis), National Institute of Public Health and the Environment (RIVM), Bilhtoven, The Netherlands
(4) Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (Lis), National Institute of Public Health and the Environment, Bilhtoven, The Netherlands

Book Title: Mycobacterium tuberculosis Protocols

Series: Methods in Molecular Medicine | Volume: 54 | Pub. Date: May-19-2001 | Page Range: 165-203 | DOI: 10.1385/1-59259-147-7:165

Subject: Microbiology

References

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