SpringerProtocols: Abstract: Angiogenesis Assays Using Chick Chorioallantoic Membrane

Angiogenesis Assays Using Chick Chorioallantoic Membrane

By: David C. West², W. Douglas Thompson³, Paula G. Sells⁴, Mike F. Burbridge⁵

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The study of the angiogenic process and the search for novel therapeutic agents to inhibit, or stimulate, angiogenesis has employed a wide range of in vivo ’angiogenesis’ assays (reviewed in 1–3). These differ greatly in their difficulty, quantitative nature, rapidity, and cost. The classical in vivo models include the rabbit ear chamber, hamster cheek pouch, dorsal skin chamber, dorsal skin and air-sac model, anterior chamber/iris and avascular corneal pocket assay, and the chick embryo chorioallantoic membrane (CAM) assay. More recent methods involve the implantation of preloaded Matrigel or alginate plugs, or collagen or poly vinyl sponges (1). Largely owing to its simplicity and low cost, the CAM is the most widely used in vivo model for the study of both angiogenesis and antiangiogenesis (1,4)

Affiliation(s): (2) Department of Immunology,Faculty of Medicine, University of Liverpool, Liverpool, UK
(3) Department of Pathology, Medical School, Aberdeen Royal Infirmary, Aberdeen, UK
(4) Alistair Reid Venom Research Unit, Liverpool School of Tropical Medicine, Liverpool, UK
(5) Experimental Oncology Division, Institut de Rechèrches Servier, Suresnes, France

Book Title: Angiogenesis Protocols

Series: Methods in Molecular Medicine | Volume: 46 | Pub. Date: Mar-16-2001 | Page Range: 107-129 | DOI: 10.1385/1-59259-143-4:107

Subject: Cell Biology

References

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