SpringerProtocols: Abstract: Entrapment of Biocatalysts by Prepolymer Methods

Entrapment of Biocatalysts by Prepolymer Methods

By: Atsuo Tanaka⁵, Takamitsu Iida⁶

Abstract

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Entrapment of biocatalysts should be carried out under mild conditions, especially when synthetic polymer materials are used as supports. In the case of photo-crosslinkable resin prepolymers having photosensitive functional groups, irradiation of near-ultraviolet (UV) light (maximum intensity at 365 nm) induces radical polymerization of the prepolymers and gel formation completes within a few minutes. Various types of prepolymers with different chain lengths, ionic properties, and/or hydrophilicity-hydrophobicity balance have been developed (1-4). The gels thus formed by photo reaction have a higher mechanical strength than those prepared by thermochemical reaction.

Affiliation(s): (5) Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan
(6) Department of Material Science and Technology, Faculty of Engineering, Niigata University, Niigata, Japan

Book Title: Enzymes in Nonaqueous Solvents: Methods and Protocols

Series: Methods in Biotechnology | Volume: 15 | Pub. Date: Mar-16-2001 | Page Range: 19-30 | DOI: 10.1385/1-59259-112-4:19

Subject: Protein Science

References

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