SpringerProtocols: Abstract: Studies of Gliadin-Specific T-Cells in Celiac Disease

Studies of Gliadin-Specific T-Cells in Celiac Disease

By: øyvind Molberg², Stephen N. McAdam², Knut E. A. Lundin³, Ludvig M. Sollid⁴

Abstract

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Celiac disease is an immune-mediated disorder that primarily affects the small intestinal mucosa. It is one of the few human disorders of which it is possible, and ethically acceptable, to obtain samples from the disease-affected tissue. This chapter describes how small intestinal biopsy specimens are utilized for studies of cell-mediated immune responses in celiac disease. The focus is mainly on practical procedures for isolation, growth under sterile conditions, and subsequent analyses of gliadin-specific T-cells derived from the small biopsy specimens. This chapter also provides guidelines for the preparation of different gliadin antigens suitable for T-cell analysis. Note that most of the T-cell assays described necessitate serological and/or genomic HLA typing of the celiac disease patients from whom the T-cells are derived.

Affiliation(s): (2) Institute of Immunology, Rikshospitalet,University of Oslo, Oslo, Norway
(3) Institute of Immunology, Rikshospitalet,University of Oslo and Department of Medicine, Ullevaal sykehus, Oslo, Norway
(4) Institute of Transplantation Immunology, Rikshospitalet,University of Oslo, Oslo, Norway

Book Title: Celiac Disease: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 41 | Pub. Date: Apr-01-2000 | Page Range: 105-124 | DOI: 10.1385/1-59259-082-9:105

Subject: Microbiology

References

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