SpringerProtocols: Abstract: Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry

Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry

By: Peter A. van Veelen², Jan W. Drijfhout², Frits Koning²

Abstract

Full Text

Download PDF (415K)

In recent years there have been major developments in the field of mass spectrometry (MS) that permit the analysis and characterization of peptides and proteins at the femtomolar level (1–8). This chapter deals with the use of MS for the elucidation of peptide sequences of gliadin- and/or glutenin-derived fragments. It is meant to give an overview of what modern mass spectrometric methods can mean for your research in the biomolecular field rather than an in-depth step-by-step practical protocol. Important aspects are covered to give the reader an idea of what is needed to perform, and what can be expected from, this mass spectrometric application.

Affiliation(s): (2) Department of Immunohaematology and Bloodbank, University of Leiden, Leiden, The Netherlands

Book Title: Celiac Disease: Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 41 | Pub. Date: Apr-01-2000 | Page Range: 75-88 | DOI: 10.1385/1-59259-082-9:075

Subject: Microbiology

References

Download References

1.	Dongre, A. R., Eng, J. K., and Yates, J. R. III (1997) Emerging tandem-mass-spectrometry techniques for the rapid identification of proteins. Trends Biotechnol. 15(10), 418–425.

2.	Yates, J. R. III (1996) Protein analysis by mass spectrometry. Methods Enzymol. 271, 351–377.

3.	Gillece-Castro, B. L. and Stults, J. (1996) Peptide characterization by mass spectrometry. Methods Enzymol. 271, 403–427.

4.	Chait, B. T. and Kent, S. B. H. (1992) Weighing naked proteins: practical, highaccuracy mass measurement of peptides and proteins. Science 257, 1885–1893.

5.	Hillenkamp, F., Karas, M., Beavis, R. C., and Chait, B. T. (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal. Chem. 63, 1193A–1203A.

6.	Fenn, J. B., Mann, M., Kai Meng, C., Fu Wong, S., and Whitehouse, C. M. (1989) Electrospray ionization for mass spectrometry of large biomolecules. Science 246, 64–71.

7.	Neubauer, G., King, A., Rappsilber, J., Calvio, C., Watson, M., Ajuh, P., Sleeman, J., Lamond, A., and Mann, M. (1998) Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. Nat. Genet. 20(1), 46–50.

8.	Den Haan, J. M., Sherman, N. E., Blokland, E., Huczko, E., Koning, F., Drijfhout, J. W., Skipper, J., Shabanowitz, J., Hunt, D. F., Engelhard, V. H., and Goulmy, E. (1995) Identification of a graft versus host disease-associated human minor histocompatibility antigen. Science 268, 1476–1480.

9.	Van de Wal, Y., Kooy, Y., van Veelen, P., Pena, S., Mearin, L., Molberg, O., Lundin, K. E. A., Sollid, L. M., Mutis, T., Benckhuijsen, W., Drijfhout, J. W., and Koning, F. (1998) Small intestinal T cells of celiac disease patients recognize a natural pepsin fragment of gliadin. Proc. Natl. Acad. Sci. USA 95, 10,050–10,058.

10.	Eng, J., McCormack, A., and Yates, J. III (1994) An approach to correlate tandem mass spectral data of peptides with amino acid sequences in protein databases. J. Am. Soc. Mass Spectrom. 5, 976–989.

11.	Mann, M. and Wilm, M. (1994) Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Anal. Chem. 66, 4390–4399.

Comments (Loading...)

Post comment/View all comments