SpringerProtocols: Abstract: 4. In Vitro Delivery of Drugs and Other Molecules to Cells

4. In Vitro Delivery of Drugs and Other Molecules to Cells

By: Marie-Pierre Rols³, Muriel Golzio³, Christine Delteil³, Justin Teissié³

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The permeability of a cell membrane can be transiently increased locally when an external electric field pulse with an overcritical intensity is applied. A position dependent modulation of the membrane potential difference is induced during the pulse. A local membrane alteration is created, which may reseal. Its molecular definition remains unknown. This phenomenon is now commonly known as electroporation or electropermeabilization. The former term implies that physical pores are created in the lipid matrix. However their existence has never been clearly demonstrated. The term electroporation is therefore rather misleading.

Affiliation(s): (3) IPBS-CNRS UPR 9062, Toulouse, France

Book Title: Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells

Series: Methods in Molecular Medicine | Volume: 37 | Pub. Date: Feb-18-2000 | Page Range: 83-97 | DOI: 10.1385/1-59259-080-2:83

Subject: Cell Biology

References

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1.	Neumann, E., Sowers, A. E., and Jordan, C. A. (1989) Electroporation and Electrofusion in cell biology. Plenum, New York.

2.	Allen, M. J., Cleary, S. F., Sowers, A. E., and Shillady, D. D. (1992) Charge and Field Effects in Biosystems, Vol. 3. Birkhaüser, Boston.

3.	Chang, D. C., Chassy, B. M., Saunders, J. A., and Sowers, A. E. (1992) Guide to Electroporation and Electrofusion. Academic Press, San Diego, CA.

4.	Belehradek, M., Domenge, C., Luboinski, B., Orlowski, S., Belehradek, J., and Mir, L. M. (1993) Electrochemotherapy, a new antitumor treatment: First clinical phase I-II trial. Cancer 72, 3694–3700.

5.	Sixou, S. and Teissié, J. (1990) Specific electropermeabilization of leucocytes in a blood sample and application to large volumes of cells. Biochim. Biophys. Acta 1028, 154–160.

6.	Titomorov, A. V., Sukharev, S., and Kistanova, E. (1991) In vivo electroporation and stable transformation fo skin cells of new born mice by plasmid DNA. Biochim. Biophys. Acta 1088, 131–134.

7.	Zeira, M., Tozi, P. F., Moumeine, Y., Lazarte, J., Sneed, L., Volsky, D. J., and Nicolau, C. (1991) Full length CD4 electroinserted in the red blood cell membrane as a long-lived inhibitor of hiv infection. Proc. Natl. Acad. Sci. USA 88, 4409–4413.

8.	Prausnitz, M. R. (1996) The effects of electric current applied to the skin: a review for transdermal drug delivery. Adv. Drug Deliv. Rev. 18, 395–425.

9.	Vanbever, R., Lecouturier, N., and Preat, V. (1994) Transdermal delivery of metoprolol by electroporation. Pharmacol. Res. 11, 1657–1662.

10.	Bernhard, J. and Pauly, H. (1973) On the generation of potential difference across the membranes of ellipsoidal cells in an alternating electric field. Biophysik 10, 89–98.

11.	Tekle, E., Astumian, R. D., and Chock, P. B. (1990) Electropermeabilization of cell membranes: effect of the resting membrane potential. Biochem. Biophys. Res. Commun. 172, 282–287.

12.	Hibino, M., Shigemori, M., Itoh, H., Nagayama, K., and Kinosita, K. (1991) Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential. Biophys. J. 59, 209–220.

13.	Schwister, K. and Deuticke, B. (1985) Formation and properties of aqueous leaks induced in human erythrocytes by electrical breakdown. Biochim. Biophys. Acta 816, 332–348.

14.	Rols, M. P. and Teissié, J. (1990) Electropermeabilization of mammalian cells. Biophys. J. 58, 1089–1098.

15.	Gabriel, B. and Teissié, J. (1997) Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane. Biophys. J. 73, 2630–2637.

16.	Kinosita, K. and Tsong, T. Y. (1977) Formation and resealing of pores of controlled sizes in human erythrocytes membrane. Nature, 268, 438–443.

17.	Gabriel, B. and Teissié, J. (1998) Fluorescence imaging in the millisecond time range of membrane electropermeabilization of single cells, using a rapid ultra low light intensifying detection system. Eur. Biophys. J. 27, 291–298.

18.	Sixou, S. and Teissié, J. (1993) Exogenous uptake and release by electroloaded cells: a digitized videomicroscopy study. Bioelectrochem. Bioenerg. 31, 237–257.

19.	Mehrle, W., Zimmermann, U., and Hampp, R. (1985) Evidence for asymmetrical uptake of fluorescent dyes through electropermeabilized membranes of avena mesophyll protoplasts. FEBS Lett. 185, 89–94.

20.	Rossignol, D. P., Decker, G. L., Lennarz, W. J., Tsong, T. Y., and Teissié, J. (1983) Induction of calcium dependent localized cortical granule breakdown in sea urchin eggs by voltage pulsation. Biochim. Biophys. Acta 763, 346–355.

21.	Dimitrov, D. S. and Sowers, A. E. (1990) Membrane electroporation-fast molecular exchange by electroosmosis. Biochim. Biophys. Acta 1022, 381–392.

22.	Teruel, M. N. and Meyer, T. (1997) Electroporation induced formation of individual calcium entry sites in the cell body and processes of adherent cells. Biophys. J. 73, 1785–1796.

23.	Teissié, J. and Rols, M. P. (1993) An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization. Biophys. J. 65, 409–413.

24.	Teissié, J. and Rols, M. P. (1994) Manipulation of cell cytoskeleton affects the lifetime of cell membrane electropermeabilization. Ann. N.Y. Acad. Sci. 720, 98–110.

25.	Rols, M. P. and Teissié, J. (1990) Modulation of electrically induced permeabilization and fusion of chinese hamster ovary cells by osmotic pressure. Biochemistry 29, 4561–4567.

26.	Rols, M. P., Dahhou, F., Mishra, K. P., and Teissié, J. (1990) Control of electric field induced cell membrane permeabilization by membrane order. Biochemistry 29, 2960–2966.

27.	Teissié, J. and Ramos, C. (1998) Correlation between electric field pulses induced long lived permeability and fusogenicity in cell membranes. Biophys. J. 74, 1889–1898.

28.	Orlowski, S. and Mir, L. M. (1993) Cell electropermeabilization: a new tool for biochemical and pharmacological studies. Biochim. Biophys. Acta 1154, 51–63.

29.	Melvik, J. E., Pettersen, E. O., Gordon, P. B., and Seglen, P. O. (1986) Increase of cis-Dichlorodiamineplatinium(II) cytotoxicity upon reversible electropermeabilization of the plasma membrane in culture human NHIK3025 cells. Eur. J. Cancer Clin. Oncol. 22, 1523–1530.

30.	Müllig, P., Forster, W., Jacob, H. E., Berg, H., Roglin, G., and Wohlrabe, K. (1984) Cell membrane permeabilization of the anthracyclines violamycin BI and BII induced by an electric field pulse. Stud. Biophys. 104, 207–213.

31.	Raffy, S. and Teissié, J. (1997) Surface charge control of electropermeabilization and glycophorin electroinsertion with 1,2-diacyl-sn-glycero-3-phosphocholine (lecithin) liposomes. Eur. J. Biochem. 250, 315–319.

32.	Gabriel, B. and Teissié, J. (1994) Generation of reactive-oxygen species induced by electropermeabilization of chinese hamster ovary cells and their consequence on cell viability. Eur. J. Biochem. 223, 25–33.

33.	Sixou, S. and Teissié, J. (1995) Electropermeabilization of peripheral blood phagocytes induces a long lived cell membrane alteration which does not affect their viability. Bioelectrochem. Bionerg. 38, 129–136.

34.	Volker, T., Pianet, I., Labouesse, J., and Teissié, J. (1989) Signal transduction by membrane receptors in viable electropermeabilized cells: Isoproterenol-stimulated cyclic AMP synthesis in C6 glioma cells. Biochim. Biophys. Acta 984, 243–251.

35.	Morgan, W. F. and Day, J. P. (1995) The introduction of proteins in mammalian cells be electroporation. Methods Mol. Biol. 48, 63–71.

36.	Liang, H., Purucker, W. J., Stenger, D. A., Kubiniec, R. T., and Hui, S. W. (1988) Uptake of fluorescence-labeled dextrans by 10 T1/2 fibroblasts following permeation by rectangular and exponential-decay electric field pulse. Biotechniques 6, 550–555.

37.	Xie, T. D. and Tsong, T. Y. (1992) Study of mechanisms of electric field-induced DNA transfection III. Electric parameters and other conditions for effective transfection. Biophys. J. 63, 28–34.

38.	Wolf, H., Rols, M. P., Neumann, E., and Teissié, J. (1994) Control by pulse parameters of electric field mediated gene transfer in mammalian cells. Biophys. J. 66, 524–531.

39.	Rols, M. P. and Teissié, J. (1998) Control by pulse duration of electropermeabilization of mammalian cells to macromolecules. Biophys. J. 75, 1415–1423.

40.	Prautsnitz, M., Milano, C., Gimm, A., and Langer, R. (1994) Quantitative study of molecular transport due to electroporation: Uptake of bovine serum albumin by erythrocytes ghosts. Biophys. J. 66, 1522–1530.

41.	Kubiniec, R.T., Liang, H., and Hui, S. W. (1990) Effect of pulse length and pulse strength on transfection by electroporation. Biotechniques, 8, 16–21.

42.	Neumann E., Kakorin, S., Tsoneva, I., Nikolova, B., and Tomov, T. (1996) Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation. Biophys. J. 71, 868–877.

43.	Hui, S. W. (1996) Effects of pulse length and strength on electroporation efficiency. Methods Mol. Biol. 55, 29–40.

44.	Rosemberg, Y. and Korenstein, R (1990) Electroporation of the photosynthetic membrane. Biophys. J. 58, 823–832.

45.	Kubiniec, R. T., Liang, H., and Hui, S. W. (1988) Use of fluorescence-labeled dextrans by 10 T1/2 fibroblasts following permeation by rectangular and exponential decay electric field pulses. Biotechniques 8, 16–20.

46.	Rols, M. P., Delteil, C., Golzio, M., Dumont, P., Cros, S., and Teissié, J. (1998) In vivo electrically mediated protein and gene transfer in murine melanoma. Nature Biotech. 16, 168–171.

47.	Kwee, S., Nielsen, H. V., Celis, J. E., Celis, A., and Madsen, P. S. (1989) Permeabilization of mammalian cells in monolayer culture by electroporation at low electric field strength. Stud. Biophys. 130, 173–176.

48.	Chu, G. H., Hayakawa, H., and Berg, P. (1987) Electroporation for the efficient expression of mammalian cells with DNA. Nucl. Acids Res. 15, 1311–1326.

49.	Potter, H. (1989) Molecular genetic applications of electroporation. Electroporation and Electrofusion in Cell Biology. (Neumann, E., Sowers, A., and Jordan, C., eds.). Plenum, New York pp. 331–342.

50.	Sukharev, S. I., Klenchin, V. A., Serov, S. M., Chernomordik, L. V., and Chizmadzhev, Y. 1992. Electroporation and electrophoretic DNA transfer into cells: The effect of DNA interaction with electropores. Biophys. J. 63, 1320–1327.

51.	Heller, R., Jaroszeski, M., Atkin, A., Moradpour, D., Gilbet, R., Wands, J., and Nicolau, C. (1996) In vivo gene electroinjection and expression in rat liver. FEBS Lett. 389, 225–228.

52.	Nishi, T., Yoshizato, K., Yamashiro, S., Takeshima, H., Sato, K., Hamada, K., Kitamura, I., Yoshimura, T., Saya, H., Kuratsu, J. I., and Ushio, Y. (1996) Highefficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Cancer Res. 56, 1050–1055.

53.	Kinosita, K. Jr., Ashikawa, I., Nobuyuki, S., Yoshimura, H., Hiroyasu, I., Nagayama, K., and Ikegami, A. (1988) Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope. Biophys. J. 53, 1015–1019.

54.	Hibino, M., Hiroyasu, I., and Kinosita, K. Jr. (1993) Time courses of cell electroporation as revealed by submicrosecond imaging of transmembrane potential. Biophys. J. 64, 1789–1800.

55.	Sugar, I. P., Forster, W., and Neumann, E. (1987) Model of cell electrofusion: Membrane electroporation, pore coalescence and percolation. Biophys. Chem. 26, 321–335.

56.	Rols, M. P., Femenia, P., and Teissié, J. (1995) Long lived macropinocytosis takes place in electropermeabilized mammalian cells. Biochem. Biophys. Res. Commun. 208, 26–38.

57.	Lambert, H., Pankow, R., Gauthier, J., and Hancock, R. (1990) Electroporationmediated uptake of proteins into mammalian cells. Biochem. Cell Biol. 68, 729–734.

58.	Glogauer, M., Lee, W., and McCulloch, A. G. (1993) Introduction of large molecules into viable fibroblasts by electroporation: Optimization of loading and identification of labelled celular compartments. Exp. Cell. Res. 208, 232–240.

59.	Rols, M. P., Dahhou, F., and Teissié, J. (1994) Pulse-first heterofusion of cells by electric field pulses and associated loading of macromolecules into mammalian cells. Biotechniques 17, 762–769.

60.	Okino, M. and Mohri, H. (1987) Effects of a high-voltage electrical impulse and an anticancer drug on in vivo growing tumors. Jpn. J. Cancer Res. 78, 1319–1321.

61.	Miklavčič, D., Beravs, K., Šemrov, D., Čemažar, M., Demšar, F., and Serša, G. (1998) The importance of electric field distribution for effective in vivo electroporation of tissues. Biophys. J. 74, 2152–2158.

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