SpringerProtocols: Abstract: 11. Mechanistic Studies of Skin Electroporation Using Biophysical Methods

11. Mechanistic Studies of Skin Electroporation Using Biophysical Methods

By: Mark R. Prausnitz³, Uwe Pliquett⁴, Rita Vanbever⁵

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The mechanism by which high-voltage pulses transiently disrupt lipid bilayers in cell membranes has been the subject of controversy since electroporation was first observed almost three decades ago. Determining the mechanism by which such pulses permeabilize the complex, multilamellar bilayer structures in skin poses an even greater challenge. To address this issue, a range of methods have been employed to perform biophysical characterization for skin electroporation studies. In this chapter, we provide an overview of these methods and highlight representative findings which biophysical characterization has yielded.

Affiliation(s): (3) Schools of Chemical Engineering and Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA
(4) University of Bielefeld, Bielefeld, Germany
(5) Department of Pharmaceutical Technology, School of Pharmacy, Catholic University of Louvain, Brussels, Belgium

Book Title: Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells

Series: Methods in Molecular Medicine | Volume: 37 | Pub. Date: Feb-18-2000 | Page Range: 213-245 | DOI: 10.1385/1-59259-080-2:213

Subject: Cell Biology

References

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