SpringerProtocols: Abstract: 9. Diagnostic Flow Cytometry in Hematologic Malignancies

9. Diagnostic Flow Cytometry in Hematologic Malignancies

By: Gerald E. Marti², Maryalice Stetler-Stevenson³, Thomas Fleisher⁴

Abstract

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During the past 30 years, we have seen flow cytometry (FCM) emerge from being a research tool requiring a group of engineers, an optical bench, and a darkened room to a benchtop flow cytometer that is used routinely in a clinical setting. The flow cytometer is to cell biology what the UV-visible spectrophotometer is to solution spectroscopy. Several events have contributed to the development of this technology to where it is now an indispensable tool in the diagnosis and clinical monitoring of disease. Many cell types are now under intense investigation. The clinical application of FCM to lymphocyte subset immunophenotyping in the leukemias and lymphomas was responsible for the early development of clinical FCM.

Affiliation(s): (2) Flow and Image Cytometry Section, Laboratory of Medical and Molecular Genetics, Division of Cell and Gene Therapies, CBER FDA NIH, Bethesda, MD
(3) Hematopathology Section, Laboratory of Pathology, National Cancer Institute, National Institute of Health, Bethesda, MD
(4) Chief, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD

Book Title: Hematologic Malignancies: Methods and Techniques

Series: Methods in Molecular Medicine | Volume: 55 | Pub. Date: Nov-30-2000 | Page Range: 179-215 | DOI: 10.1385/1-59259-074-8:179

Subject: Molecular Medicine

References

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