SpringerProtocols: Abstract: Raf-1 Protein Kinase Activity in T Cells from Aged Mice

Raf-1 Protein Kinase Activity in T Cells from Aged Mice

By: Christopher J. Kirk², Richard A. Miller³

Abstract

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Most of our models of signal transduction through the T-cell receptor (TCR) involve components and pathways first described in T-cell clones and T-cell lymphomas such as the Jurkat cell line (1). These studies, while providing valuable insights, are not always reliable guides to the analogous biochemical events in cells freshly isolated from live donors (2). Thus, studies of the effects of aging on T-cell activation must frequently begin with a detailed study of the responses of cells from young individuals. Studies of aging effects involve additional challenges, including the difficulty of purifying sufficient numbers of cells from specific subsets, and allowing for the inherent variability among donors of any age.

Affiliation(s): (2) Graduate Program in Cellular and Molecular Biology, University of Michigan Institute of Gerontology and Ann Arbor DVA Medical Center, Ann Arbor, MI
(3) Department of Pathology, University of Michigan Institute of Gerontology and Ann Arbor DVA Medical Center, Ann Arbor, MI

Book Title: Aging Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 38 | Pub. Date: May-26-2000 | Page Range: 85-96 | DOI: 10.1385/1-59259-070-5:85

Subject: Molecular Medicine

References

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