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Assays for Kinesin Microtubule-Stimulated AT Pase Activity
| Abstract |
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Methods for assay of ATPase rates can be classified into broad categories depending on whether an ATP-regenerating system
or a continuous coupled assay is employed versus quenching at discrete times for subsequent analysis for production of Pi
or ADP (or, more rarely, for the loss of ATP).
Affiliation(s): (2) Department of Biological Science, Carnegie Mellon University, Pittsburgh, PA
(3) Department of Biological Science, Carnegie Mellon University, Pittsburgh, PA
(3) Department of Biological Science, Carnegie Mellon University, Pittsburgh, PA
Book Title: Kinesin Protocols
Series: Methods in Molecular Biology | Volume: 164 | Pub. Date: Dec-03-2000 | Page Range: 65-71 | DOI: 10.1385/1-59259-069-1:65
Subject: Protein Science
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