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Assays for Microtubule-Destabilizing Kinesins
Abstract
The kinesin superfamily is a large family of microtubule (MT)-stimulated ATPases that couple the energy of ATP hydrolysis to force production (reviewed in refs. 1 and 2). Conventional kinesin was identified as a motor protein that could translocate along MT polymers. Since the molecular cloning of kinesin (3), well over 100 additional proteins have been identified that share high sequence homology with the catalytic domain of kinesin. Many of these kinesin-related proteins (KRPs) have also been shown to be MT-based motor proteins using in vitro motility assays (reviewed in refs. 2, see also chapter 5, chapter 8, and chapter 12).
Affiliation(s): (2) Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
(3) Medical Sciences Program, Indiana University, Bloomington, IN
Book Title: Kinesin Protocols
Series: Methods in Molecular Biology  |  Volume: 164  |  Pub. Date: Dec-03-2000  |  Page Range: 109-121  |  DOI: 10.1385/1-59259-069-1:109
Subject:  Protein Science
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