SpringerProtocols: Abstract: The E. coli Vsr Endonuclease: Assaying Activity In Vitro and In Vivo

The E. coli Vsr Endonuclease: Assaying Activity In Vitro and In Vivo

By: Claire G. Cupples², Georgina Macintyre²

Abstract

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The primary function of the Vsr endonuclease of Escherichia coli is to initiate repair of T/G mismatches that arise from deamination of 5-methylcytosine to thymine (reviewed in 1). By making a nick 5′ of the T, Vsr provides a site for DNA polymerase I to start the removal of the mismatched base, along with several nucleotides on its 3′ side. Resynthesis of the missing bases by the polymerase restores the correct C.G basepair and completes the process of very short patch (VSP) repair.

Affiliation(s): (2) Department of Biology, Concordia University, Montréal, Québec, Canada

Book Title: DNA Repair Protocols: Prokaryotic Systems

Series: Methods in Molecular Biology | Volume: 152 | Pub. Date: Jul-07-2000 | Page Range: 63-73 | DOI: 10.1385/1-59259-068-3:63

Subject: Genetics/Genomics

References

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