SpringerProtocols: Abstract: 7. Cytokinins: Extraction, Separation, and Analysis

7. Cytokinins: Extraction, Separation, and Analysis

By: Paula E. Jameson², Huaibi Zhang³, David H. Lewis⁴

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Analysis of cytokinins in plant tissues has historically been laborious and time consuming because of the extremely low levels and diverse molecular structures of the cytokinins. The initial anticipation that cytokinins could be quantified by radioimmunoassay of crude plant extracts (1,2) was, unfortunately, not justified. Detailed analysis requires the sample to be well purified and the individual cytokinins to be separated, so that not only is interference in the assay avoided, but also problems associated with differential crossreactivity of individual cytokinins with the antibodies in the immunoassay (3). The (sometimes subtle) differences in the chemical properties of the cytokinin free bases, ribosides, nucleotides, and the O- and N-glucosides enables the ready separation and quantification of the majority of the cytokinins.

Affiliation(s): (2) Institute of Molecular BioSciences, College of Sciences, Massey University, Palmerston North, New Zealand
(3) The Horticulture and Food Research Institute of New Zealand, Palmerston North, New Zealand
(4) New Zealand Institute for Coop and Food Research, Palmerston North, New Zealand

Book Title: Plant Hormone Protocols

Series: Methods in Molecular Biology | Volume: 141 | Pub. Date: Mar-17-2000 | Page Range: 101-121 | DOI: 10.1385/1-59259-067-5:101

Subject: Plant Sciences

References

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1.	Weiler, E. W. (1979) Radioimmunoassay for the determination of free and conjugated abcisicacid.Planta 144, 255–263.

2.	Weiler, E. W. (1980) Radioimmunoassays for trans-zeatin and related cytokinins. Planta 149, 155–162.

3.	Hedden, P. (1993) Modern methods for the quantitative analysis of plant hormones. Annu. Rev. Plant Physiol. Plant Mol. Bol. 44, 107–129.

4.	Jameson, P. E. (1994) Cytokinin metabolism and compartmentation,in Cytokinins: Chemistry, Activity and Function (Mok, D. W. S. and Mok, M. C., eds.), CRC, Boca Raton, FL, pp. 113–128.

5.	Tokota, T., Murofushi, N., and Takahashi, N. (1985) Extraction, purification,and identification, in Hormonal Regulation of Development I: Molecular Aspects of Plant Hormones (MacMillan, J., ed.),Springer-Verlag, Berlin, pp. 113–201.

6.	Parker, C. W. and Letham, D. S. (1973) Regulators of cell division in plant tissues XVI. Metabolism of zeatin by radish cotyledons and hypocotyls. Planta 114, 199–218.

7.	Morris, J. W., Doumas, P., Morris, R. O., and Zaerr J. B. (1990) Cytokinins in vegetative and reproductive buds of Pseudotsuga menziesii. Plant Physiol. 93, 67–71.

8.	Dietrich, J. T., Kaminek, M., Blevins, D. G., Reinbott, T. M., and Morris, R. O. (1995) Changes in cytokinins and cytokinin oxidase activity in developing maize kernels and the effects of exogenous cytokinin on kernel development. Plant Physiol. Biochem. 33, 327–336.

9.	Bieleski, R. L. (1964) The problem of halting enzyme action when extracting plant tissues. Anal. Biochem. 9, 431–442.

10.	Horgan, R. and Scott, I. M. (1987) Cytokinins, in Principles and Practices of Plant Hormone Analysis (Rivier, L. and Crozier, A., eds.),Academic, New York, pp. 303–365.

11.	Summons, R. E., Palni, L. M. S., and Letham, D. S. (1983) Determination of intact zeatin nucleotide by direct chemical ionisation mass spectrometry. FEBS Lett. 151, 122–126.

12.	Meilan, R., Horgan, R., Heald, J. K., LaMotte, C. E., and Schultz, R. C. (1993) Identification of cytokinins in red pine seedlings. Plant Growth Regul. 13, 169–178.

13.	von Schwartzenberg, K., Bonnet-Masimbert, M., and Doumas, P. (1994) Isolation of two cytokinin metabolites from the rhizosphere of Norway spruce seedlings (Picea abies L. Karst.). Plant Growth Regul. 15, 117–124.

14.	Jameson, P. E., Letham, D. S., Zhang, R., Parker, C. W., and Badenoch-Jones, J.(1987) Cytokinin translocation and metabolism in Lupinspecies. I Zeatin riboside introduced into the xylem at the base of Lupinus angustifolius stems.Aust. J. Plant Physiol. 14, 695–718.

15.	Wang, J., Letham, D. S., Taverner, E., Badenoch-Jones, J., and Hocart, C. H. (1995) A procedure for quantification of cytokinins as free bases involving scintillation proximity immunoassay. Physiol.Plant. 95, 91–98.

16.	Palmer, M. V., Horgan, R., and Wareing, P. F. (1981) Cytokinin metabolism in Phaseolus vulgaris L.: Identification of endogenous cytokinins and metabolism of [8-¹⁴C] dihydrozeatin in stems of decapitated plants. Planta 153, 297–302.

17.	Muller, P. and Hilgenberg, W. (1986) Isomers of zeatin and zeatin riboside in clubroot tissue: evidence for trans-zeatin biosynthesis by Plasmodiophora brassicae. Physiol. Plant. 66, 245–250.

18.	Cappeiello, P. E. and King, G. J. (1990) Determination of zeatin and zeatin riboside in plant tissue by solid-phase extraction and ion-exchange chromatography. J. Chromatog. 504, 197–201.

19.	Biddington, N. L. and Thomas, T. H. (1976) Effect of pH on the elution of cytokinins from polyvinylpyrrolidone columns. J. Chromatog. 121, 107–109.

20.	Mousdale, D. M. A. and Knee, M. (1979) Poly-N-vinylpyrrolidone column chromatography of plant hormones with methanol as eluent.J. Chromatog. 177, 398–400.

21.	Parker, C. W. and Letham, D. S. (1974) Regulators of cell division in plant tissues XVII. Metabolism of zeatin in Zea mays seedlings.Planta 115, 337–344.

22.	Badenoch-Jones, J., Letham, D. S., Parker, C. W., and Rolfe, B. G. (1984) Quantitation of cytokinins in biological samples using antibodies against zeatin riboside. Plant Physiol. 75, 1117–1125.

23.	Guinn, G. and Brummett, D. L. (1990) Solid phase extraction of cytokinins from aqueous solutions with C₁₈ cartridges and their use in a rapid purification procedure. Plant Growth Regul. 9, 305–314.

24.	MacDonald, E. M. S. and Morris, R. O. (1985) Isolation of cytokinins by immunoaffinity chromatography and analysis by high-performance liquid chromatography-radioimmunoassay. Methods Enzymol. 110, 347–358.

25.	Morris, R. O., Zaerr, J. B., and Chapman, R. W. (1976) Trace enrichment of cytokinins from Douglas-fir xylem exudate. Planta 131, 271–274.

26.	Jameson, P. E. and Morris, R. O. (1989) Zeatin-like cytokinins in yeast: Detection by immunological methods. J. Plant Physiol. 135, 385–390.

27.	Hocart, C. H., Letham, D. S., and Parker, C. W. (1990) Metabolism and translocation of exogenous zeatin riboside in germinating seeds and seedlings of Zea mays. J. Exp. Bot. 41, 1517–1524.

28.	Lewis, D. H., Burge, G. K., Schmierer, D. M., and Jameson, P. E.(1996) Cytokinins and fruit development in the kiwifruit (Actinidia deliciosa). I. Changes during fruit development. Physiol. Plant. 98, 179–186.

29.	Banowetz, G. M. (1994) Immunoanalysis of cytokinins, in Cytokinins: Chemistry, Activity and Function (Mok, D. W. S. and Mok, M. C., eds.), CRC, Boca Raton, FL, pp. 305–316.

30.	Eason, J. R., Morris, R. O., and Jameson, P. E. (1996) The relationship between virulence and cytokinin production by Rhodococcus fascians (Tilford 1936) Goodfellow 1984. Plant Pathol. 45, 323–331.

31.	Pengelly, W. L. (1986) Validation of immunoassays, in Plant Growth Substances 1985 (Bopp, M., ed.), Springer-Verlag, Berlin, pp. 35–43.

32.	Prinsen, E., Redig, P., Van Dongen, W., Esmans, E. L., and Van Onckelen, H. A. (1995) Quantitative analysis of cytokinins by electrospray tandem mass spectrometry. Rapid Commun. Mass Spectrom. 9, 948–953.

33.	MacDonald, E. M., Akiyoshi, D. E., and Morris, R. O. (1981) Combined high-performance liquid chromatography-radioimmunoassay for cytokinins. J. Chromatog. 214, 101–109.

34.	Trione, E. J., Krygier, B. B., Banowetz, G. M., and Kathrein, J. M. (1985) The development of monoclonal antibodies against the cytokinin zeatin riboside. J. Plant Growth Regul. 4, 101–109.

35.	Trione, E. J., Krygier, B. B., Kathrein, J. M., Banowetz, G. M., and Sayavedra-Soto, L. A. (1987) Monoclonal antibodies against the plant cytokinin isopentenyl adenosine. Physiol. Plant. 70, 467–472.

36.	Dawson, R. M. C., Elliott, D. C., Elliott, W. H., and Jones, K. M. (1986) Ion Exchange, gel filtration, and affinity chromatography media. In: Data for Biochemical Research, 3rd ed., Clarendon Press, Oxford, pp. 508–513.

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