SpringerProtocols: Abstract: 10. Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing

10. Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing

By: Mary Ellexson-Turner², David Sidebottom², Sean Turner², Tara Bennett², William H. Hildebrand²

Abstract

Full Text

Download PDF (125K)

Class I human leukocyte antigen (HLA-I) alleles have been described as themost polymorphic human antigens (1-4). To date, there are more than 500 HLA- I alleles which have been officially assigned by the World Health Organization Nomenclature Committee for Factors of the HLA System (5). This number continues to grow, with approximately one new HLA-I allele reported weekly (6).

Affiliation(s): (2) Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK

Book Title: Antigen Processing and Presentation Protocols

Series: Methods in Molecular Biology | Volume: 156 | Pub. Date: Sep-15-2000 | Page Range: 129-142 | DOI: 10.1385/1-59259-062-4:129

Subject: Immunology

References

Download References

1.	Hedrick, P. W., Whittman, T. S., and Parham, P. Amino acid variation at HLA-A and HLA-B: High parallel polymorphism but high differentiation in selected haplotypes. Amer. J. Hum. Genet., submitted.

2.	Lawlor, D. A., Ward, F. E., Ennis, P. D., Jackson, A. P., and Parham, P. (1988). HLA-A,B polymorphisms predate the divergence of humans and chimpanzees. Nature 335, 268–271.

3.	Nei, M. and Jin, L. (1989) Variances of the average numbers of nucleotide substitutions within and between populations. Mol. Biol. Evol. 6, 290–300.

4.	Parham, P. (1988) Function and polymorphism of human leukocyte antigen-A,B,C molecules. Amer. J. Med. 85, (suppl. 6A), 2–5.

5.	Bodmer, J. G., Marsh, S. G. E., Albert, E. D., Bodmer, W. F., Bontrop, R. E., Dupont, B., et al. (1999). Nomenclature for factors of the HLA system, 1998. Tissue Antigens 53, 407–446.

6.	Parham, P., Adams, E. J., and Arnett, K. L. (1995) Origins of HLA-A, B, and C polymorphism. Immunol. Rev. 143, 141–180.

7.	Grumet, F. C., Payne, R. O., Konishi, J., and Kriss, J. P. (1974) HL-A antigens as markers for disease susceptibility and autoimmunity in Graves’ disease. J. Clin. Endocrinol. Metab. 39, 1115–9.

8.	Payne, R., Perkins, H. A., Kountz, S. L., and Belzer, F. O. (1971) Unrelated kidney transplants and matching for HL-A antigens. Transplant. Proc. 3, 1036–1041.

9.	Perkins, H. A., Kountz, S. L., Payne, R., and Belzer, F. O. (1971) Achievements and limitations of histocompatibility testing for ten HL-A factors in kidney trans-plantation. Transplant Proc. 3, 130–2.

10.	Darke, C. (1983) HLA-B 12 and-B40 cross-reactive groups and their serological relationships. Tissue Antigens 21, 281–295.

11.	Darke, C. (1983) Inclusion of HLA-Bw42, Bw54 and Bw55 within the HLA-B14 cross-reactive group. Tissue Antigens 21, 246–253.

12.	Darke, C. (1983) Reanalysis of the HLA-B7 cross-reactive group. Tissue Antigens 22, 326–334.

13.	Darke, C. (1984) Serological study of the HLA-B17 cross-reactive group. Tissue Antigens 23, 141–150.

14.	Hildebrand, W. H., Domena, J. D., and Parham, P. (1993) Primary structure shows HLA-B59 to be a hybrid of HLA-B55 and HLA-B51, and not a variant of HLA-B8. Tissue Antigens 41, 190–195.

15.	Hildebrand, W. H., Madrigal, J. A., Belich, M. P., Zemmour, J., Ward, F. E., Williams R. C., and Parham, P. (1992) Serologic cross-reactivities poorly reflect allelic relationships in the HLA-B12 and HLA-B21 groups: dominant epitopes of the α₂ helix. J. Immunol. 149, 3563–3568.

16.	Madrigal, J. A., Hildebrand, W. H., Belich, M. P., Benjamin, R. J., Little, A.-N., Zemmour, J. et al. (1993) Structural diversity in the HLA-A10 family of alleles: Correlations with serology. Tissue Antigens 41, 72–78.

17.	Parham, P. (1992) Typing for Class I HLA Polymorphism: Past, present and future. Eur. J. Immunogen. 19, 347–359.

18.	Zemmour, J., Gumperz, J. E., Hildebrand, W. H., Ward, F. E., Marsh, S. G. E., Williams, R. C., and Parham, P. (1992) Molecular basis for reactivity of anti-Cw1 and anti-Cw3 alloantisera with HLA-B46 haplotypes. Tissue Antigens 39, 249–257.

19.	Parham, P., Adams, E. J., and Arnett, K. L. (1995) Origins of HLA-A,B,C Polymorphism. Immunol. Rev. 143, 141–180.

20.	Turner, S., Ellexson, M. E., Hickman, H. D., Sidebottom, D. A., Fernandez-Vina, M., Confer, D. L. and Hildebrand, W. H. (1998) Sequence-based typing provides a new look at HLA-C diversity. J Immunol. 161, 1406–1413.

Comments (Loading...)

Post comment/View all comments