SpringerProtocols: Abstract: 6. DNA Sequencing by Capillary Array Electrophoresis

6. DNA Sequencing by Capillary Array Electrophoresis

By: Norman J. Dovichi⁵, JianZhong Zhang⁶

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On May 9,1998, PE Biosystems announced that they would introduce a new instrument based on “breakthrough DNA analysis technology” (1). Over 700 orders for the new sequencer were received in the first 5 mo of production, which make this instrument one of the most successful new products in the history of analytical instrumentation. This new instrument is based on capillary array electrophoresis (CAE) with a sheath-flow cuvet detector. This detection technology provides nearly 100% duty cycle, where every capillary is monitored simultaneously without the need for a scanning detector system. By use of this instrument, the Drosophila melanogaster sequence will be completed in less than 5 mo (2), and the sequence of the human genome will be substantially complete by the time that this book is in print.

Affiliation(s): (5) Department of Chemistry, University of Washington, Seattle, WA
(6) Molecular Dynamics, Sunnyvale, CA

Book Title: Capillary Electrophoresis of Nucleic Acids: Volume I: Introduction to the Capillary Electrophoresis of Nucleic Acids

Series: Methods in Molecular Biology | Volume: 162 | Pub. Date: Dec-27-2000 | Page Range: 85-94 | DOI: 10.1385/1-59259-055-1:85

Subject: Biochemistry

References

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