SpringerProtocols: Abstract: 5. Microchip-Based Capillary Electrophoresis Systems

5. Microchip-Based Capillary Electrophoresis Systems

By: Wenzhang Xie⁵, Rong Yang⁵, Junquan Xu⁵, Liang Zhang⁵, Wanli Xing⁵, Jing Cheng^{6 7}

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Microchip-based capillary electrophoresis (CE) systems have enjoyed impressive advancement in the past few years (1–3). Up until now, CE array chips have been fabricated based upon the well-understood behavior of a single channel chip system. Different materials (e.g., silicon [4], glass [5], and plastics [6]) have been used. A variety of different fabrication processes have also been developed to accommodate the complicated requirements and materials used for making such a device. Microchip-based CE systems have demonstrated uses in diverse applications such as the separation of amino acids (7), analysis of blood serum cortisol (8), examination of polymerase chain reaction (PCR) amplicons (9), and analysis of metal ion complexes (10). In this chapter, aspects related to chip-based CEs will be reviewed.

Affiliation(s): (5) Aviva Biosciences Corporation, San Diego, CA
(6) Biochip Research and Development Center, School of Life Sciences and Engineering, Tsinghua University, Beijing, China
(7) Biochip Research and Development Center, State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China

Book Title: Capillary Electrophoresis of Nucleic Acids: Volume I: Introduction to the Capillary Electrophoresis of Nucleic Acids

Series: Methods in Molecular Biology | Volume: 162 | Pub. Date: Dec-27-2000 | Page Range: 67-83 | DOI: 10.1385/1-59259-055-1:67

Subject: Biochemistry

References

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