SpringerProtocols: Abstract: Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins

Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins

By: Jean-Louis Schwartz², Luke Masson³

Abstract

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The availability of bacterial toxin genes and, in many cases, their atomic structures permits, through properly designed structure-function studies, the precise mapping of the molecular determinants of their activity. The results of such studies are of particular importance for the elucidation of the mode of action of the toxins and the design of genetically engineered molecules displaying novel activity. For example, a large effort is currently underway to design, through mutagenesis and chimeric constructs, the next generation of Bacillus thuringiensis toxin-based pesticides with different specificities, improved toxicity, and the potential to delay pest resistance (1).

Affiliation(s): (2) Biotechnology Research Institute,National Research Council,and Groupe de Recherche en Transport Membranaire, Université de Montréal, Montréal, Canada
(3) Biotechnology Research Institute, National Research Council Canada, Montréal, Canada

Book Title: Bacterial Toxins: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 145 | Pub. Date: Mar-10-2000 | Page Range: 101-113 | DOI: 10.1385/1-59259-052-7:101

Subject: Infectious Diseases

References

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