This chapter defines the terms and examines the configurations used for most applications of ELISA. Such a chapter is important
because the possibilities inherent in the systems of ELISA must be understood in order to maximize their versatility in assay
design. All heterogeneous systems have three basic parameters:
| 1. |
One reactant is attached to a solid phase, usually a plastic microtiter plate with an 8 × 12-well format. |
| 2. |
Separation of bound and free reagents, which are added subsequently to the solid phase-attached substance, is by a simple
washing step.
|
| 3. |
Results are obtained through the development of color. |
Series: Methods in Molecular Biology | Volume: 149 | Pub. Date: Aug-17-2000 | Page Range: 9-44 | DOI: 10.1385/1-59259-049-7:9
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