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The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection
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The amino acids we have the most experience with are GABA, glutamate and arginine, though other amino acids can be measured.
The method used is based on a derivatization reported by Jacobs (1) using o-phthalaldehyde in the presence of sulphite. The method was used by Pearson et al. (2), Smith and Sharp (3), and was further developed by Rowley et al. (4).
Affiliation(s): (2) School of Biomedical Sciences, Nottingham University Medical School, Queen’s Medical Centre, Nottingham, UK
Book Title: Amino Acid Analysis Protocols
Series: Methods in Molecular Biology | Volume: 159 | Pub. Date: Sep-05-2000 | Page Range: 55-62 | DOI: 10.1385/1-59259-047-0:055
Subject: Biochemistry
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