SpringerProtocols: Abstract: Generation of HLA Class I Transfected Target Cell Lines

Generation of HLA Class I Transfected Target Cell Lines

By: Jenny E. Gumperz³

Abstract

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Normal human lymphoblastoid cell lines (LCLs) express six classical class I HLA genes from three gene loci, HLA-A, HLA-B, and HLA-C, which are located within the major histocompatibility complex (MHC). Because of the extensive allelic polymorphism of HLA class I genes, cell lines from heterozygous individuals can express up to six different class I alleles (1). Class I phenotypes, as well as other properties of different lymphoblastoid target cells, can therefore be very heterogeneous. Hence, in investigating the effects on and specificity of class I recognition by natural killer (NK) cells, it has proved useful to use transfected target cells that express only one class I allele, so that the effects of individual class I allotypes can be compared directly using the same target cell background.

Affiliation(s): (3) Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women′s Hospital and Harvard Medical School, Boston, MA

Book Title: Natural Killer Cell Protocols: Cellular and Molecular Methods

Series: Methods in Molecular Biology | Volume: 121 | Pub. Date: Feb-04-2000 | Page Range: 49-60 | DOI: 10.1385/1-59259-044-6:49

Subject: Immunology

References

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