SpringerProtocols: Abstract: Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Multiplex Polymerase Chain Reaction

Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Multiplex Polymerase Chain Reaction

By: Ian G. Wilson²

Abstract

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Since 1983, when Escherichia coli O157:H7 was first recognised as a cause of hemorrhagic colitis and hemolytic uremic syndrome (HUS), verotoxin-producing strains of E. coli (VTEC) have been identified as the cause of increasing numbers of cases of serious human illness (1,2). One of a number of large outbreaks in Scotland resulted in 20 deaths, and a massive outbreak in Japan affected more than 9000 people (3,4). In England and Wales, 90 % of VTEC cases are sporadic (5).

Affiliation(s): (2) Bacteriology Department, Belfast City Hospital,Belfast, Northern Ireland

Book Title: Food Microbiology Protocols

Series: Methods in Biotechnology | Volume: 14 | Pub. Date: Dec-12-2000 | Page Range: 85-94 | DOI: 10.1385/1-59259-029-2:85

Subject: Microbiology

References

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