SpringerProtocols: Abstract: Construction of Mouse Adenovirus Type 1 Mutants

Construction of Mouse Adenovirus Type 1 Mutants

By: Angela N. Cauthen², Katherine R. Spindler²

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Construction of mouse adenovirus type 1 (MAV-1) mutants is facilitating studies of adenoviral pathogenesis in the natural host. We have isolated the first site-directed viral mutants of MAV-1, and we are comparing effects of wild-type (wt) and these mutant viruses in infections of mice (Smith et al., submitted; Ying et al., submitted; refs. 1 and 2). The mutants have lesions in viral genes early region 1 (E1) or 3 (E3), and have altered lethality in adult mice.

Affiliation(s): (2) Department of Genetics, University of Georgia, Athens, GA

Book Title: Adenovirus Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 21 | Pub. Date: Aug-28-1998 | Page Range: 85-103 | DOI: 10.1385/0-89603-551-4:85

Subject: Microbiology

References

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