SpringerProtocols: Abstract: Large-Scale Purification and Crystallization of Adenovirus Hexon

Large-Scale Purification and Crystallization of Adenovirus Hexon

By: John J. Rux², Donatelia Pascolini², Roger M. Burnett³

Abstract

Full Text

Download PDF (1403K)

Adenovirus, shortly after its discovery in 1953 (1), was one of the first biological entities to be imaged using the electron microscope (EM) (2). Since then, It has been under continuous scrutiny by structural biologists (reviewed inref. 3). The characteristic shape of the adenovirus virion, with its projecting fibers, made it a fascinating object for early EM studies, which focused on how its > 1O structural proteins are organized to form the virion. The size of the virion, with a diameter of 914Ã¥ at the fivefold vertices (4) and a mass of over 150 Ã 1O⁶ Dalton, presents a formidable challenge for X-ray crystallographic studies.

Affiliation(s): (2) Department of Biochemistry/Sciences II, University of Geneva, Switzerland
(3) The Wistar Institute, Philadelphia, PA

Book Title: Adenovirus Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 21 | Pub. Date: Aug-28-1998 | Page Range: 259-275 | DOI: 10.1385/0-89603-551-4:259

Subject: Microbiology

References

Download References

1.	Rowe, W. P., Huebner, R. J., Gillmore, L. K., Parrott, R. H., and Ward, T. G. (1953) Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Sot Exp Biol Med 84, 570–573.

2.	Horne, R. W., Brenner, S., Waterson, A. P., and Wildy, P. (1959) The icosahedral form of an adenovirus J Mol Biol 1, 84–86.

3.	Burnett, R. M (1996) The structure of adenovirus, in Structural Biology of Viruses (Chiu, W., Burnett, R. M., and Garcea, R., eds.), Oxford University Press, NewYork, pp.209–238.

4.	Stewart, P. L., Burnett, R. M., Cyrklaff, M., and Fuller, S. D. (1991) Image reconstruction reveals the complex molecular organization of adenovirus. Cell 67, 145–154.

5.	White, D. O., Scharff, M. D., and Maizel, J. V., Jr. (1969) The polypeptides of adenovirus III Synthesis in infected cells Virology 38, 395–406.

6.	Pereira, H. G., Valentine, R. C., and Russell, W. C. (1968) Crystallization of an adenovirus protein (the hexon) Nature 219, 946,947.

7.	Burnett, R. M., Grutter, M. G., and White, J. L. (1985) The structure of the aden-ovnus capsid. I An envelope model of hexon at 6$$A resolution JMolBiol 185, 105–123.

8.	Roberts, M M., White, J. L., Grutter, M. G., and Burnett, R. M. (1986) Three-dimensional structure of the adenovirus maJor coat protein hexon. Science 232, 1148–1151.

9.	Athappilly F. K. Murah R. Rux J. J. Cal Z. and Burnett R. M. 1994 Therefined crystal structure of hexon the major coat protein of adevirus type 2 at29 Ã¥ resolution. J Mol Biol 242, 430–455

10.	van Oostrum, J., and Burnett, R. M. (1985) Molecular composition of the adenovirus type 2 virion. J Virol 56, 449–448.

11.	Burnett, R. M. (1985) The structure of the adenovirus capsid II The packing symmetry of hexon and its implications for viral architecture. J Mol. Biol 185, 125–143.

12.	Stewart P. L. Fuller S. D. and Burnett R. M. (1993) Difference imaging of adenovirus. Bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO J 12 2589–2599)

13.	Fureinitti, P. S., van Oostrum, J., and Burnett, R. M. (1989) Adenovirus polypeptide IX revealed as capsid cement by difference images from electron microscopy and crystallography EMBO J 8, 3563–3570.

14.	Kozarsky, K. F., and Wilson, J. M. (1993) Gene therapy. adenovirus vectors Curr Opin Genet Dev 3, 499–503.

15.	Franklin, R. M., Petterson, U., Ã¥kervall, K., Strandberg, B., and Philipson, L.(1971) Structural proteins of adenovirus V Size and structure of the adenovirus type 2 hexon. J MolBiol 57, 383–395.

16.	Cormck, G., Sigler, P. B., and Ginsberg, H. S. (1971) Characterization of crystals of type 5 adenovirus hexon. J. Mol Biol 57, 397–401.

17.	Dohner, L., and Hudemann, H. (1972) Untersuchungen zur kristallisation von Hexonen der Adenoviren der Gruppe III Arch Gesamte Virusforsch 38, 279–289.

18.	Hay, R. T., personal communication.

19.	Devaux, C., Adrian, M., Berthet-Colominas, C., Cusack, S., and Jacrot, B. ( 1990)Structure of adenovirus fibre. I. Analysis of crystals of fibre from adenovirus serotypes 2 and 5 by electron microscopy and X-ray crystallography J Mol Biol 215, 567–588.

20.	Xia, D., Henry, L. J., Gerard, R. D., and Deisenhofer, J. (1994) Crystal structure of the receptor-binding domain of adenovirus type 5 fiber protein at 1.7Ã¥ resolution Structure 2, 1259–1270.

21.	Schoehn G. Fender P. Chroboczek J. and Hewat E. A. 1996 Adevirus 3 penton dodecahedron exhibits structural changes of the base on fibre binding EMBO J. 15 6841–6846

22.	Cepko C. L. and Sharp P. A. 1982 Assembly of adevirus maJor capsid protein is mediated by a nvirion protein Cell 31 407–415

23.	Pettersson, U., Philipson, L., and Hoglund, S. (1967) Structural proteins ofadenoviruses I Purification and characterization of the adenovirus type 2 hexon antigen. Virology 33, 575–590.

24.	Dowdle, W. R., Lambriex, M., and Hierholzer, J. C. (1971) Production and evaluation of a purified adenovirus group-specific (hexon) antigen for use in the diagnostic complement fixation test. Appl Microbiol 21, 718–722.

25.	Boulanger, P. A., and Puvton, F. (1973) Large-scale preparation of soluble adenovirus hexon, penton and fiber antigens in highly purified form Eur J Biochem 39, 37–42.

26.	Grutter, M., and Franklin, R. M. (1974) Studies on the molecular weight of the adenovirus type 2 hexon and its subunit. J Mol Biol 89, 163–178.

27.	Jornvall, H., Pettersson, U., and Phthpson, L. (1974) Structural studies of adenovirus type-2 hexon protein Eur J Biochem 48, 179–192.

28.	Siegel, S. A., Hutchms, J. E., and Witt, D. J. (1987) Purification of adenovirus hexon by high performance liquid chromatography. J Virol Methods 17, 211–217.

29.	Challberg, S. S., and Ketner, G. (1981) Deletion mutants of adenovirus 2. Isolation and initial characteitzation of virus carrying mutations near the right end of the viral genome Virology 114, 196–209.

30.	McPherson, A. (1982) Preparation and analysis of protein crystals. Wiley, New York

31.	Owens, J., Walthall, B., and Murphy, T. (1993) The effects of freshly prepared cell culture medium vs stored cell culture medium on high-density cell culture Amer Biotech Lab 11, 64–66.

Comments (Loading...)

Post comment/View all comments