SpringerProtocols: Abstract: Biosensors based on DNA intercalation using light polarization

Biosensors based on DNA intercalation using light polarization

By: John J. Horvath³

Abstract

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The intercalation of polyaromatic compounds by DNA can serve as a basis for a simple and sensitive method for detection and quantification of carcinogens. The experimental technique is based on monitoring the decrease of polarization, caused by the displacement of an intercalated fluorescent dye molecule by the analyte molecule (carcinogen). The magnitude of the polarization decrease is proportional to the concentration of the analyte.

Affiliation(s): (3) Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD

Book Title: Affinity Biosensors: Techniques and Protocols

Series: Methods in Biotechnology | Volume: 7 | Pub. Date: Apr-17-1998 | Page Range: 161-171 | DOI: 10.1385/0-89603-539-5:161

Subject: Biotechnology

References

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