SpringerProtocols: Abstract: Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems

Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems

By: F. Peter Guengerich³, Natalie A. Hosea³, Martha V. Martin³

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A procedure is outlined that is used for purification of heterologously expressed P4503A4 from Escherichia coli membranes. Details of constructron of the particular plasmid expressed (NF14) are presented elsewhere (1, 2). The general procedure can be utilized for other cytochromes P450, with some modification, as described elsewhere (1, 3–7). Several general purification techniques are described in detarl in Chapters 4 and 5 of this volume.

Affiliation(s): (3) Department of Biochemistry, Vanderbilt University, Nashville, TN

Book Title: Cytochrome P450 Protocols

Series: Methods in Molecular Biology | Volume: 107 | Pub. Date: Jul-30-1998 | Page Range: 77-84 | DOI: 10.1385/0-89603-519-0:77

Subject: Biochemistry

References

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