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Measurement of Agonist-Stimulated [35S]GTPγS Binding to Cell Membranes
Abstract
Abstract This chapter describes a functional assay that measures the increase in guanine nucleotide exchange at G proteins in cell membranes, resulting from agonist binding to G protein-coupled receptors (GPCRs), by monitoring the binding of a radiolabeled, hydrolysis-resistant analog of GTP, [35S]GTPγS, in the presence of unlabeled GDP.
Affiliation(s): (2) MRC Collaborative Centre, Mill Hill, London, UK
Series: Methods in Molecular Biology  |  Volume: 83  |  Pub. Date: May-08-1997  |  Page Range: 107-116  |  DOI: 10.1385/0-89603-495-X:107
Subject:  Neuroscience
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