SpringerProtocols: Abstract: Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis

Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis

By: Ulla Wienhues², Wolfgang Kraas², Christoph Seidel², Hans Georg Ihlenfeidt², Günther Jung²

Abstract

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This chapter focuses on methods for epitope mapping on novel viral polypeptrdes and on fine tuning sensitivity and spectficity of the identified peptide antigens for application in virus diagnosis. Because of the development of efficient methods of multiple peptrde synthesis (1–3), antigenic sates of many proteins could be detected

Affiliation(s): (2) Boehringer Mannheim, Tutzing, Germany

Book Title: Molecular Diagnosis of Infectious Diseases

Series: Methods in Molecular Medicine | Volume: 13 | Pub. Date: Dec-18-1997 | Page Range: 125-142 | DOI: 10.1385/0-89603-485-2:125

Subject: Infectious Diseases

References

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4.	Ihlenfeldt H-G, Seidel C, Wienhues U, and Jung G. (1997) Complete Epitope Fine-Mapping of the Hepatitis C Virus-Polyprotein Manuscript submitted

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