SpringerProtocols: Abstract: Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers

Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers

By: Isabelle Rivière², Michel Sadelain³

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Retroviral vectors are powerful tools for gene transfer that are useful in the context of experimental as well as clinical applications. Defective recombinant retroviruses allow for efficient gene transfer into a broad range of mammalian cells derived from different species and different tissues. Safe gene transfer can be achieved using helper-free viral stocks, owing to the development of vectors and packaging cell lines that minimize the probability of hazardous recombination events. Retrovirus-mediated gene transfer leads to stable proviral integration in the target-cell genome, a key feature when the foreign genetic material must be faithfully transmitted to the progeny of the transduced parental cell, murine leukemia virus (MLV)-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.

Affiliation(s): (2) New York University Medical Center, New York
(3) Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York

Book Title: Gene Therapy Protocols

Series: Methods in Molecular Medicine | Volume: 7 | Pub. Date: Oct-01-1996 | Page Range: 59-78 | DOI: 10.1385/0-89603-484-4:59

Subject: Genetics/Genomics

References

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