SpringerProtocols: Abstract: Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors

Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors

By: Jeffrey S. Bartlett², Richard J. Samulski²

Abstract

Full Text

Download PDF (1645K)

The development of gene transfer vectors from the human parvovirus, adeno-associated virus (AAV), has provided scientists with an efficient and effective way of delivering genes into mammalian cells. This chapter aims to explore the various practical aspects of the AAV vector system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology involved in the generation of recombinant AAV vectors, it is of value to briefly discuss the structure and life cycle of this unique virus. Detailed and more extensive reviews that describe the biology of adeno-associated virus are also available (1-3).

Affiliation(s): (2) Gene Therapy Center, University of North Carolina, Chapel Hill, NC

Book Title: Gene Therapy Protocols

Series: Methods in Molecular Medicine | Volume: 7 | Pub. Date: Oct-01-1996 | Page Range: 25-40 | DOI: 10.1385/0-89603-484-4:25

Subject: Genetics/Genomics

References

Download References

1.	Bartlett, J. S and Samulski, R. J. (1995) Genetics and biology of adeno-associated virus, in Viral Vectors Gene Therapy and Neuroscience Applications (Kaplitt, M. G. and Loewy, A. D., eds.), Academic, San Diego, pp. 55–73.

2.	Cukor, G., Blacklow, N R., Hoggan, D, and Berns, K. I (1984) Biology of adeno-associated virus, in The Parvoviruses (Berns, K. I., ed), Plenum, New York, pp. 33–66.

3.	Samulski, R. J. (1993) Adeno-associated virus: integration at a specific chromopsomal locus. Curr Opin Gen Dev. 3, 74–80.

4.	Berns, K. I. and Adler, S. (1972) Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains J Virol 5, 693–699.

5.	Berns, K. I. and Rose, J A. (1970) Evidence for a single-stranded adeno-associated virus genome isolation and separation of complementary single strands. J Virol 5, 693–699.

6.	Blacklow, N. R., Hoggan, M. D., and Rowe, W. P. (1967) Isolation of adenovirus-associated viruses from man Proc Natl Acad. Sci. USA 58, 1410–1415.

7.	Mayor, H D., Torikai, K, Melnick, J., and Mandel, M (1969) Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions. Science 166, 1280–1282.

8.	Samulski, R J., Chang, L.-S., and Shenk, T. (1987) A recombinant plasmid from which an infectious adeno-associated virus genome can be excised in vitro and its use to study viral replication. J. Virol 61, 3096–3101.

9.	Srivastava, A., Lusby, E W., and Berns, K. I (1983) Nucleotide sequence and organization of the adeno-associated virus 2 genome J Virol. 45, 555–564.

10.	Lusby, E., Fife, K H., and Berns, K. I. (1980) Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA. J Virol 34, 402–409.

11.	Muzyczka, M. (1992) Use of adeno-associated virus as a general transduction vector for mammalian cells. Curr Top Microbiol Immunol. 158, 97–129.

12.	Berns, K. I. (1990) Parvorviridae and their replication, in Virology, vol 2., 2nd ed. (Fields, B. N. and Knipe, D. M., eds.), Raven, New York, pp 1743–1763.

13.	Berns, K. I and Huaswirth, W. W (1984) Adeno-associated virus DNA structure and replication, in The Parvoviruses (Berns, K I., ed), Plenum, New York, p 1.

14.	Hermonat, P L. and Muzyezka, N (1984) Use of adeno-associated virus as a mammalian DNA cloning vector transduction of neomycin resistance into mammalian tissue culture cells. Proc. Natl. Acad. Sci USA 81, 6466–6470.

15.	McLaughlin, S. K., Collis, P., Hermonat, P. L., and Muzyczka, N. (1988) Adeno-associated virus general transduction vectors: analysis of proviral structures J Virol 62, 1963–1973.

16.	Samulski, R. J., Chang, L.-S., and Shenk, T. (1989) Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 63, 3822–3828.

17.	Atchison, R. W., Casto, B. C., and Hammond, W. M (1965) Adenovirus-associated defective virus particles. Science 149, 754–756.

18.	Hoggan, M. D, Blacklow, N. R., and Rowe, W P. (1966) Studies of small DNA viruses found in various adenovirus preparations physical, biological, and immunological characteristics Proc Natl Acad Sci. USA 55, 1457–1471.

19.	Parks, W P., Melnick, J. L., Rongey, R., and Mayor, H. D (1967) Physical assay and growth cycle studies of a defective adeno-satellite virus J Virol 1, 171–180.

20.	Buller, R. M., Janik, J. E., Sebring, E. D., and Rose, J. A (1981) Herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication. J Virol 40, 241–247.

21.	Berns, K. I., Pinkerton, T. C., Thomas, G. F., and Hoggan, M. D. (1975) Detection of adeno-associated virus (AAV)-specific nucleotide sequences in DNA isolated from latently infected Detroit 6 cells. Virology 68, 556–560.

22.	Cheung, A. K., Hoggan, M. D., Hauswirth, W. W., and Berns, K. I (1980) Integration of the adeno-associated virus genome into cellular DNA in latently infected human Detroit 6 cells. J. Virol. 33, 739–748.

23.	Laughlin, C. A., Tratschin, J.-D., Coon, H., and Carter, B. J. (1983) Cloning of infectious adeno-associated virus genomes in bacterial plasmids Gene 23, 65–73.

24.	Samulski, R. J., Berns, K. I., Tan, M., and Muzyczka, N. (1982) Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells Proc Natl. Acad Sci USA 79, 2077–2081.

25.	Bartlett, J. S., Quattrocchi, K. B., and Samulski, R. J (1995) The development of adeno-associated virus as a vector for cancer gene therapy, in The Internet Book of Gene Therapy Cancer Therapeutics (Sobol, R. E and Scanlon, K. J, eds), Appleton & Lange, Stamford, CT, pp 27–40.

26.	LaFace, D., Hermonat, P., Wakeland, E, and Peck, A (1988) Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector Virology 162, 483–486.

27.	Ohi, S, Dixit, M., Tillery, M. K., and Plonk, S G. (1990) Construction and replication of an adeno-associated virus expression vector that contains human β-globin cDNA Gene 89, 279–282.

28.	Tratschin, J-D., West, M. H. P, Sandbank, T., and Carter, B. J (1984) A human parvovirus, adeno-associated virus, as a eukaryotic vector transient expression and encapsidation of the prokaryotic gene for chloramphenicol acetyltransrerase Mol Cell Biol 4, 2072–2081.

29.	Kumar, S. and Leffak, M. (1991) Conserved chromatin structure in c-myc 5′ flanking DNA after viral transduction. J. Mol. Biol. 222, 45–57.

30.	Walsh, C. E, Liu, J. M, Xiao, X, Young, N. S, Nienhuis, A W., and Samulski, R. J. (1992) Regulated high level expression of a human λ-globin gene introduced into erythroid cells by an adeno-associated virus vector Proc Natl Acad Sci USA 89, 7257–7261.

31.	Goodman, S., Xiao, X., Donahue, R. E., Moulton, A, Miller, J, Walsh, C, Young, N. S, Samulski, R J., and Nienhuis, A W. (1994) Recombinant adeno-associated virus-mediated gene transfer into hematopoietic progenitor cells. Blood 84, 1492–1500.

32.	Walsh, C. E., Nienhuis, A. W., Samulski, R. J, Brown, M. G, Miller, J. L., Young, N. S., and Liu, J. M. (1994) Phenotypic correction of Fanconi anemia in human hematopoietic cells with a recombinant adeno-associated virus vector. J. Clin Invest. 94, 1440–1448.

33.	Kriegler, M (1990) Gene Transfer and Expression A Laboratory Manual Stockton, New York.

34.	Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) Molecular Cloning A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

35.	Felgner, P. L. and Ringold, G. M. (1989) Cationic liposome-mediated transfection. Nature 337, 387, 388.

Comments (Loading...)

Post comment/View all comments