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3. Amplification of DNA Sequences Up To 5 kb from Small Amounts of Genomic DNA Using Tub DNA Polymerase
Abstract
Standard PCR protocols can be used to amplify sequences of a few hundred base pairs in length from the DNA of a single cell (1,2). However, the efficiency of standard PCR amplification using Taq DNA polymerase declines with sequence length, making it difficult to amplify long sequences from small amounts of genomic DNA.
Affiliation(s): (2) University of California, San Francisco, CA
Series: Methods in Molecular Biology  |  Volume: 67  |  Pub. Date: Oct-01-1996  |  Page Range: 31-38  |  DOI: 10.1385/0-89603-483-6:31
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