| 1. |
Uhlén, M., Forsberg, G., Moks, T., Hartmanis, M., and Nilsson, B. (1992) Fusion proteins in biotechnology. Curr. Opin. Biotechnol.
3, 363–369.
|
| |
| 2. |
StÃ¥hl, S., Nygren, P.-Ã
., Sjolander, A., and Uhlén, M. (1993) Engineered bacterial receptors in immunology. Curr. Opin. Immunol.
5, 272–277.
|
| |
| 3. |
Nygren, P.-Ã
., Ståhl, S., and Uhlén, M. (1994) Engineering proteins to facilitate bioprocessing. Trends Biotechnol.
12, 184–188.
|
| |
| 4. |
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1988) Molecular Cloning A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
|
| |
| 5. |
Wang, A. M. and Mark, D. (1990) PCR Protocols A Guide to Methods and Applications (Innis M. A., Gelfand, D. H., Sninsky, J. J., and White, T. J., eds.), Academic, San Diego, CA.
|
| |
| 6. |
Mullis, K. and Faloona, F. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol.
155, 335–350.
|
| |
| 7. |
Ståhl, S., Hansson, M., Ahlborg, N., Nguyen, T. N., Liljeqvist, S., Lundeberg, J., and Uhlén, M. (1993) Solid-phase gene assembly
of constructs derived from the Plasmodium falciparum malaria blood-stage antigen Ag332. BioTechniques
14, 424–434.
|
| |
| 8. |
Nguyen, T. N., Uhlén, M., and StÃ¥hl, S. (1994) De novo gene assembly using a paramagnetic solid support, in Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, Φ., eds.), Eaton, Natick, MA, pp. 73–78.
|
| |
| 9. |
Hornes, E., Hultman, T., Moks, T., and Uhlén, M. (1990) Direct cloning of the human genomic apolipoprotein E gene using magnetic
separation of single-stranded DNA. BioTechniques
9, 730–737.
|
| |
| 10. |
Uhlén, M. and Moks, T. (1990) Gene fusions for purpose of expression: an introduction. Methods Enzymol.
185, 129–143.
|
| |
| 11. |
Abrahmsén, L., Moks, T., Nilsson, B., and Uhlén, M. (1986) Secretion of heterologous gene products to the culture medium of
Escherichia coli. Nucleic Acids Res.
14, 7487–7500.
|
| |
| 12. |
Suominen, I., Karp, M., Lahde, M., Kopio, A., Glumoff, T., Meyer, P., and Mantsala, P. (1987) Extracellular production of
cloned a-amylase by Escherichia coli. Gene
61, 165–176.
|
| |
| 13. |
Moks, T., Abrahmsén, L., Osterlof, B., Josephson, S., Ostling, M., Enfors, S.-O., Persson, I., Nilsson, B., and Uhlén, M.
(1987) Large-scale affinity purification of human insulin-like growth factor I from culture medium of Escherichia coli. Bio/Technol.
5, 379–382.
|
| |
| 14. |
Kitai, K., Kudo, T., Nakamura, S., Masegi, T., Ichikawa, Y., and Horikoshi, K. (1988) Extracellular production of human immunoglobulin
G Fc region (hIgG-Fc) by Escherichia coli. Appl. Microbiol. Biotechnol.
28, 52–56.
|
| |
| 15. |
Obukowicz, M. G., Turner, M. A., Wong, E. Y., and Tacon, W. C. (1988) Secretion and export of IGF-1 in Escherichia coli strain JM101. Mol. Gen. Genet.
215, 19–25.
|
| |
| 16. |
Thomas, W. D., Jr., Wagner, S. P., and Welch, R. A. (1992) A heterologous membrane protein domain fused to the C-terminal
domain of HlyB can export Escherichia coli hemolysin. J. Bacteriol.
174, 771–779.
|
| |
| 17. |
Nilsson, B., Abrahmsén, L., and Uhlén, M. (1985) Immobilization and purification of enzymes with staphylococcal protein. A
gene fusion vectors. EMBO J.
4, 1075–1080.
|
| |
| 18. |
Ward, E. S., Gussow, D., Griffiths, A. D., Jones, P. T., and Winter, G. (1989) Binding activities of a repertoire of single
immunoglobulin variable domains secreted from Escherichia coli. Nature
341, 544–546.
|
| |
| 19. |
Carter, P., Kelley, R. F., Rodrigues, M. L., Snedecor, B., Covarrubias, M., Velligan, M. D., Wong, W. L. T., Rowland, A. M.,
Kotts, C. E., Carver, M. E., Yang, M., Bourell, J. H., Shepard, H. M., and Henner, D. (1992) High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Bio/Technol.
10, 163–167.
|
| |
| 20. |
Martineau, P., Charbit, A., Leclerc, C., Werts, C., O’Callaghan, D., and Hofnung, M. (1991) A genetic system to elicit and
monitor antipeptide antibodies without peptide synthesis. Bio/Technol.
9, 170–172.
|
| |
| 21. |
Murby, M., Cedergren, L., Nilsson, J., Nygren, P.-Ã
., Hammarberg, B., Nilsson, B., Enfors, S.-O., and Uhlén, M. (1991) Stabilization
of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy. Biotechnol. Appl. Biochem.
14, 336–346.
|
| |
| 22. |
Hansson, M., Ståhl, S., Hjorth, R., Uhlén, M., and Moks, T. (1994) Single-step recovery of a secreted recombinant protein
by expanded bed adsorption. Bio/Technol.
12, 285–288.
|
| |
| 23. |
Sjolander, A., Ståhl, S., Lovgren, K., Hansson, M., Cavelier, L., Walles, A., Helmby, H., Wåhlm, B., Morein, B., Uhlén, M.,
Berzins, K., Perlmann, P., and Wahlgren, M. (1993) Plasmodium falciparum. The immune response in rabbits to the clustered asparagine-rich protein (CARP) after immunization in Freund’s adjuvant or
immunostimulating complexes (ISCOMs). Exp. Parasitol.
76, 134–145.
|
| |
| 24. |
Rudolph, R. (1995) Successful protein folding on an industrial scale, in Principles and Practice of Protein Folding. (Cleland, J. L. and Craik, C. S., eds.), Wiley, New York, pp. 283–298.
|
| |
| 25. |
Ford, C. F., Souminen, I., and Glatz, C. E. (1991) Fusion tails for the recovery and purification of recombinant proteins.
Protein Expr. Purif.
2, 95–107.
|
| |
| 26. |
Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression
of cloned genes. Methods Enzymol.
185, 60–89.
|
| |
| 27. |
Murby, M., Nguyen, T. N., Binz, H., Uhlén, M., and Ståhl, S. (1994) Production and recovery of recombinant proteins of low
solubility, in Separations for Biotechnology 3 (Pyle, D. L., ed.), Bookcraft, Bath, UK, pp. 336–344.
|
| |
| 28. |
Charbit, A., Sobczak, E., Michel, M.-L., Molla, A., Tiollais, P., and Hofnung, M. (1987) Presentation of two epitopes of the
preS2 region of hepatitis B virus on live recombinant bacteria. J. Immunol.
139, 1658–1664.
|
| |
| 29. |
Agterberg, M., Adriaanse, H., Lankhof, H., Meloen, R., and Tommassen, J. (1990) Outer membrane PhoE protein of Escherichia coli as a carrier for foreign antigenic determinants: immunogenicity of epitopes of foot-and-mouth disease virus. Vaccine
8, 85–91.
|
| |
| 30. |
Pistor, S. and Hobom, G. (1988) Expression of viral hemagglutinin on the surface of E. coli. Klin. Wochenschr.
66, 110–116.
|
| |
| 31. |
Harrison, J. L., Taylor, I. M., and O’Connor, C. D. (1990) Presentation of foreign antigenic determinants at the bacterial
cell surface using the TraT lipoprotein. Res. Microbiol.
141, 1009–1012.
|
| |
| 32. |
Fuchs, P., Breitling, F., Dubel, S., Seehaus, T., and Little, M. (1991) Targeting recombinant antibodies to the surface of
Escherichia coli. Fusion to a peptidoglycan associated lipoprotein. Bio/Technol.
9, 1369–1372.
|
| |
| 33. |
Kornacker, M. G. and Pugsley, A. P. (1990) The normally periplasmic enzyme β-lactamase is specifically and efficiently translocated
through the Escherichia coli outer membrane when it is fused to the cell-surface enzyme pullulanase. Molec. Microbiol.
4, 1101–1109.
|
| |
| 34. |
Hedegaard, L., and Klemm, P. (1989) Type 1 fimbriae of Escherichia coli as carriers of heterologous antigenic sequences. Gene
85, 115–124.
|
| |
| 35. |
Newton, S. M. C., Jacob, C. O., and Stocker, B. A. D. (1989) Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science
244, 70–72.
|
| |
| 36. |
Georgiou, G., Poetschke, H. L., Stathopoulos, C., and Francisco, J. A. (1993) Practical applications of engineering Gram-negative
bacterial cell surfaces. Trends Biotechnol.
11, 6–10.
|
| |
| 37. |
Leclerc, C., Charbit, A., Martineau, P., Deriaud, E., and Hofnung, M. (1991) The cellular location of a foreign B cell epitope
expressed by recombinant bacteria determines its T cell-independent or T cell-dependent characteristics. J. Immunol.
147, 3545–3552.
|
| |
| 38. |
Francisco, J. A., Campbell, R., Iverson, B. L., and Georgiou, G. (1993) Production and fluorescence-activated cell sorting
of Escherichia coli expressing a functional antibody fragment on the external surface. Proc. Natl. Acad. Sci. USA
90, 10,444–10,448.
|
| |
| 39. |
Clackson, T. and Wells, J. A. (1994) In vitro selection from protein and peptide libraries. Trends Biotechnol.
12, 173–184.
|
| |
| 40. |
Little, M., Fuchs, P., Breitling, F., and Dubel, S. (1993) Bacterial surface presentation of proteins and peptides an alternative
to phage technology? Trends Biotechnol.
11, 3–5.
|
| |
| 41. |
Bradbury, A., Persic, L., Werge, T., and Cattaneo, A. (1993) Use of living columns to select specific phage antibodies. Bio/Technol.
11, 1565–1569.
|
| |
| 42. |
Francisco, J. A., Earhart, C. F., and Georgiou, G. (1992) Transport and anchoring of β-lactamase to the external surface of
Escherichia coli. Proc. Natl. Acad. Sci. USA
89, 2713–2717.
|
| |
| 43. |
Francisco, J. A., Stathopoulos, C., Warren, R. A. J., Kilburn, D. G., and Georgiou, G. (1993) Specific adhesion and hydrolysis
of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Bio/Technol.
11, 491–495.
|
| |
| 44. |
Hammarberg, B., Nygren, P.-Ã
., Holmgren, E., Elmblad, A., Tally, M., Hellman, U., Moks, T., and Uhlén, M. (1989) Dual affinity
fusion approach and its use to express recombinant insulin-like growth factor II. Proc. Natl. Acad. Sci. USA
86, 4367–4371.
|
| |
| 45. |
Capon, D. J., Chamow, S. M., Mordenti, J., Marsters, S. A., Gregory, T., Mitsuya, H., Byrn, R. A., Lucas, C., Wurm, F. M.,
Gioopman, J. E., Broder, S., and Smith, D. H. (1989) Designing CD4 immunoadhesins for AIDS therapy. Nature
337, 525–531.
|
| |
| 46. |
Nygren, P.-Ã
., Flodby, P., Andersson, R., Wigzell, H., and Uhlén, M. (1991) In vivo stabilization of a human recombinant CD4 derivative by fusion to a serum-albumin-binding receptor, in Vaccines 91 (Chanock, R. M., Ginsberg, H. S., Brown, F., and Lerner, R. A., eds.) Cold Spring Harbor Laboratory, Cold Spring Harbor,
NY, pp. 363–368.
|
| |
| 47. |
Samuelsson, E., Wadensten, H., Hartmanis, M., Moks, T., and Uhlen, M. (1991) Facilitated in vitro refolding of human recombinant insulin-like growth factor. I using a solubilizing fusion partner. Bio/Technol.
9, 363–366.
|
| |
| 48. |
Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T. A., and Uhlén, M.
(1987) A synthetic IgG-binding domain based on staphylococcal protein A. Proc. Eng.
1, 107–113.
|
| |
| 49. |
Samuelsson, E., Moks, T., Nilsson, B., and Uhlén, M. (1994) Enhanced in vitro refolding of insulin-like growth factor I using a solubilizing fusion partner. Biochemistry
33, 4207–4211.
|
| |
| 50. |
Uhlén, M., Nilsson, B., Guss, B., Lindberg, M., Gatenbeck, S., and Philipson, L. (1983) Gene fusion vectors based on the gene
for staphylococcal protein A. Gene
23, 369–378.
|
| |
| 51. |
Flaschel, E. and Friehs, K. (1993) Improvement of downstream processing of recombinant proteins by means of genetic engineering
methods, in Biotechnology Advances (Moo-Young, M. and Glick, B. R., eds.), Pergamon, Oxford, UK, pp. 31–78.
|
| |
| 52. |
Nilsson, B. and Abrahmsén, L. (1990) Fusions to staphylococcal protein A. Methods Enzymol.
185, 144–161.
|
| |
| 53. |
Nilsson, J., Nilsson, P., Williams, Y., Pettersson, L., Uhlén, M., and Nygren, P.-Ã
. (1994) Competitive elution of protein
A fusion proteins allows specific recovery under mild conditions. Eur. J. Biochem.
224, 103–108.
|
| |
| 54. |
Dalbøge, H., Dahl, H.-H. M., Pedersen, J., Hansen, J. W., and Christensen, T. (1987) A novel enzymatic method for production
of authentic hGH from an Escherichia coli produced hGH-precursor. Bio/Technol.
5, 161–164.
|
| |
| 55. |
Kohler, K., Ljungquist, C., Kondo, A., Veide, A., and Nilsson, B. (1991) Engineering proteins to enhance their partitioning
coefficients in aqeous two-phase systems. Bio/Technol.
9, 642–646.
|
| |
| 56. |
Lundeberg, J., Wahlberg, J., and Uhlén, M. (1990) Affinity purification of specific DNA fragments using a lac repressor fusion
protein. Gene Anal. Techn.
7, 47–53.
|
| |
| 57. |
Ljungquist, C., Lundeberg, J., Rasmussen, A.-M., Hornes, E., and Uhlén, M. (1993) Immobilization and recovery of fusion proteins
and B-lymphocyte cells using magnetic separation. DNA Cell Biol.
12, 191–197.
|
| |
| 58. |
Lundeberg, J., Wahlberg, J., and Uhlén, M. (1991) Rapid colorimetric quantification of PCR-amplified DNA. BioTechniques
10, 68–75.
|
| |
| 59. |
Yun, Z., Lundeberg, J., Johansson, B., Hedrum, A., Welland, O., Uhlén, M., and Sonnerborg, A. (1994) Colorimetric detection
of competitive PCR products for quantification of hepatitis C viremia. J. Virol. Meth.
47, 1–14.
|
| |
| 60. |
Sano, T., Smith, C., and Cantor, C. R. (1992) Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA
conjugates. Science
258, 120–122.
|
| |
| 61. |
StÃ¥hl, S., Sjolander, A., Nygren, P.-Ã
., Berzins, K., Perlmann, P., and Uhlén, M. (1989) A dual expression system for the
generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum malaria antigen Pf155/RESA. J. Immunol. Meth.
124, 43–52.
|
| |
| 62. |
Sjolander, A., Ståhl, S., and Perlmann, P. (1993) Bacterial expression systems based on protein A and protein G designed for
the production of immunogens applications to Plasmodium falciparum malaria antigens. Immunomethods
2, 79–92.
|
| |
| 63. |
Smith, G. P. (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.
Science
228, 1315–1317.
|
| |
| 64. |
Scott, J. K. and Smith, G. P. (1990) Searching for peptide ligands with an epitope library. Science
249, 386–390.
|
| |
| 65. |
Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, A. D., and Winter, G. (1991) By-passing immunization
Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol.
222, 581–597.
|
| |
| 66. |
Nissim, A., Hoogenboom, H. R., Tomlinson, I. M., Flynn, G., Midgley, C., Lane, D., and Winter, G. (1994) Antibody fragments
from a’ single pot’ phage display library as immunochemical reagents. EMBO J.
13, 692–698.
|
| |
| 67. |
Lowman, H. B. and Wells, J. A. (1993) Affinity maturation of human growth hormone by monovalent phage display. J. Mol. Biol.
234, 564–578.
|
| |
| 68. |
Martin, F., Tonatti, C., Salvati, A. L., Venturini, S., Ciliberto, G., Cortese, R., and Solazzo, M. (1994) The affinity-selection
of a minibody polypeptide inhibitor of human interleukin-6. EMBO J.
13, 5303–5309.
|
| |