SpringerProtocols: Abstract: Inducible Expression Cassettes in Yeast: ADH2

Inducible Expression Cassettes in Yeast: ADH2

By: Virginia L. Price²

Abstract

Full Text

Download PDF (1812K)

The ADH2 promoter belongs to a class of promoters subject to catabolite repression. Many of these promoters have strong transcriptional start signals, which are repressed by the presence of glucose. The strength of the ADH2 promoter coupled with the fact that the promoter is tightly regulated make it highly amenable for use in the expression of heterologous genes.

Affiliation(s): (2) Immunex Corporation, Seattle, WA

Book Title: Recombinant Gene Expression Protocols

Series: Methods in Molecular Biology | Volume: 62 | Pub. Date: Mar-06-1997 | Page Range: 149-157 | DOI: 10.1385/0-89603-480-1:149

Subject: Genetics/Genomics

References

Download References

1.	Denis, C. L. and Young, E. T. (1983) Isolation and characterization of the positive regulatory gene ADR1 from Saccharomyces cerevisiae. Mol. Cell Biol. 3, 360–310.

2.	Denis, C. L., Ciriacy, M., and Young, E. T. (1981) A positive regulatory gene is required for accumulation of the functional messenger RNA for the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J. Mol. Biol. 148, 355–368.

3.	Ciriacy, M. (1975) Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II Two loci controlling synthesis of the glucose-repressible ADHII. Mol. Gen. Genet. 138, 157–164.

4.	Ciriacy, M. (1977) Isolation and characterization of mutants defective in intermediary carbon metabolism and in carbon catabolite repression. Mol. Gen. Genet. 154, 213–220.

5.	Denis, C. L. (1984) Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108, 833–844.

6.	Yu, J., Donoviel, M. S., and Young, E. T. (1989) Adjacent upstream activation sequence elements synergistically regulate transcription of ADH2 in Saccharomyces cerevisiae. Mol. Cell Biol. 9, 34–42.

7.	Eisen, A., Taylor, W. E., Blumberg, H., and Young, E. T. (1988) The yeast regulatory protein ADR1 binds in a zinc-dependent manner to the upstream activating sequence of ADH2. Mol. Cell Biol. 8, 4552–4556.

8.	Beier, D. R. and Young, E. T. (1982) Characterization of a regulatory region upstream of the ADR2 locus of S. cerevisiae. Nature 300, 724–728.

9.	Beier, D. R., Sledziewski, A., and Young, E. T. (1985) Deletion analysis identifies a region, upstream of the ADH2 gene of Saccharomyces cerevisiae, which is required for ADR1-mediated derepression. Mol. Cell Biol. 5, 1743–1749.

10.	Russell, D. W., Smith, M., Cox, D., Williamson, V. M., and Young, E. T. (1983) DNA sequences of two yeast promoter-up mutants. Nature 304, 652–654.

11.	Ciriacy, M. (1979) Isolation and characterization of further cis-and transacting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII). Mol. Gen. Genet. 176, 427–431.

12.	Thukral, S. K., Eisen, A., and Young, E. T. (1991) Two monomers of yeast transcription factor ADR1 bind a palindromic sequence symmetrically to activate ADH2 expression. Mol. Cell. Biol. 11, 1566–1577.

13.	Cook, W. J. and Denis, C. L. (1993) Identification of three genes required for the glucose-dependent transcription of the yeast transcriptional activator ADR1. Curr. Genet. 23, 192–200.

14.	Denis, C. L. and Gallo, C. (1986) Constitutive RNA synthesis for the yeast activator ADR1 and identification of the ADR1-5 ^c mutation implications in posttranslational control of ADR1. Mol. Cell. Biol. 6, 4026–4030.

15.	Denis, C. L., Fontaine, S. C., Chase, D., Kemp, B. E., and Bemis, L. T. (1992) ADR1^c mutations enhance the ability of ADR1 to activate transcription by a mechanism that is independent of effects on cyclic AMP-dependent protein kinase phosphorylation of Ser-230. Mol. Cell. Biol. 12, 1507–1514.

16.	Cook, W. J., Chase, D., Audino, D. C., and Denis, C. L. (1994) Dissection of the ADR1 protein reveals multiple, functionally redundant activation domains interspersed with inhibitory regions: evidence for a repressor binding to the ADR1^c region. Mol. Cell. Biol. 14, 629–640.

17.	Denis, C. L. (1987) The effects of ADR1 and CCR1 gene dosage on the regulation of the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. Mol. Gen. Genet. 208, 101–106.

18.	Cherry, J. R. and Denis, C. L. (1989) Overexpression of the yeast transcriptional activator ADR1 induces mutation of the mitochondrial genome. Curr. Genet. 15, 311–317.

19.	Price, V. L., Taylor, W. E., Clevenger, W., Worthington, M., and Young, E. T. (1990). Expression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter. Methods Enzymol. 185, 308–318.

20.	Price, V., Mochizuki, D., March, C. J., Cosman, D., Deeley, M. C., Klinke, R., Clevenger, W., Gillis, S., Baker, P., and Urdal, D. (1987) Expression, purification and characterization of recombinant murine granulocyte-macrophage colony stimulating factor and bovine IL-2 from yeast. Gene 55, 287–293.

21.	Kaslow, D. C., Hui, G., and Kumar, S. (1994) Expression and antigenicity of Plasmodium falciparum major merozoite surface protein (MSP₁₉) variants secreted from Saccharomyces cerevisiae. Mol. Biochem. Parasitol. 63, 283–289.

22.	Kaslow, D. C. and Shiloach, J. (1994) Production, purification and immunogenicity of a malaria transmission-blocking vaccine candidate TBV25H expressed in yeast and purified using nickel-NTA agarose. Biotechnology 12, 494–499.

23.	Bishop, P. D., Teller, D. C., Smith, R. A., Lasser, G. W., Gilbert, T., and Seale, R. L. (1990) Expression, purification, and characterization of human factor XIII in Saccharomyces cerevisiae. Biochemistry, 29, 1861–1869.

24.	Russell, D. W. and Smith, M. (1983) Nucleotide sequence of the yeast alcohol dehydrogenase II gene. J. Biol. Chem. 258, 2674–2682.

25.	Hill, J. E., Myers. A. M., Koerner, T. J., and Tzagoloff, A. (1986) Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2, 163–167.

26.	Brunelli, J. P. and Pall, M. L. (1993) A series of yeast/Escherichia coli 1 expression vectors designed for directional cloning of cDNAs and cre/lox-mediated plasmid excision. Yeast 9, 1309–1318.

27.	Sathe, G. M., O’Brien, S., McLaughlin, M. M., Watson, F., and Livi, G. P. (1991) Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells. Nucleic Acids Res. 19, 4775.

28.	Barnes, W. M. (1994) PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc. Natl. Acad. Sci. USA 91, 2216–2220.

29.	Gearing, D. P., Thut, C. J., VandenBos, T., Gimpel, S. D., Delaney, P. B., King, J., Price, V., Cosman, D., and Beckmann, M. P. (1991) Leukemia inhibitory factor receptor is structurally related to the IL-6_signal transducer, gp130. EMBO J. 10, 2839–2848.

30.	Curtis, B. M., Williams, D. E., Broxmeyer, H. E., Dunn, J., Farrah, T., Jeffery, E., Clevenger, W., deRoos, P., Martin, U., Friend, D., Craig, V., Gayle, R., Price, V., Cosman, D., March, C. J., and Park, L. S. (1991) Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein. Proc. Natl. Acad. Sci. USA 88, 5809–5813.

31.	Adams, S. E., Dawson, K. M., Gull, K., Kingsman, S. M., and Kingsman, A. J. (1987) The expression of hybrid HIV Ty virus-like particles in yeast. Nature 329, 68–70.

32.	Chinery, S. A. and Hinchliffe, E. (1989) A novel class of vector for yeast transformation. Curr. Genet. 16, 21–25.

Comments (Loading...)

Post comment/View all comments