SpringerProtocols: Abstract: Pulsed Field Gel Electrophoresis for Mycobacteria

Pulsed Field Gel Electrophoresis for Mycobacteria

By: Wolfgang J. Philipp², Stephen Gordon³, Amalio Telenti², Stewart T. Cole⁴

Abstract

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The introduction of pulsed field gel electrophoresis (PFGE) was a major breakthrough in the field of genome research (1), allowing the analysis of whole procaryotic and eucaryotic chromosomes. This became possible by a combination of new techniques for the extraction of intact chromosomal DNA in agarose blocks and electrophoresis using varying electrical fields.

Affiliation(s): (2) Institute of Medical Microbiology, University of Berne, Berne, Switzerland
(3) Unitè de Gènètique Molèculaire Bactèrienne, Institut Pasteur, Paris, France
(4) Unite de Genetique Mycobacterienne, Departement de Bacteriologie et Mycologie, Institut Pasteur, Paris, France

Book Title: Mycobacteria Protocols

Series: Methods in Molecular Biology | Volume: 101 | Pub. Date: Aug-18-1998 | Page Range: 51-63 | DOI: 10.1385/0-89603-471-2:51

Subject: Microbiology

References

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