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Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity
Abstract
The polyamines spermine and spermidine can be metabolized by polyamine oxidases in two ways, oxidative determination or oxidative cleavage (1,2) (see Chapter 1). An example of the former is the amine oxidase present in bovine (and other ruminant) sera (3) that deaminates polyamines in accordance with Fig. 1. In contrast, tissue polyamine oxidase (typified by rat liver polyamine oxidase) (4) cleaves spermine or spermidine with the release of an aminopropyl moiety (Fig. 2).
Affiliation(s): (2) Vascular Biology Research Center, King’s College, London, UK
Series: Methods in Molecular Biology  |  Volume: 79  |  Pub. Date: Oct-10-1997  |  Page Range: 105-108  |  DOI: 10.1385/0-89603-448-8:105
Subject:  Biochemistry
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