SpringerProtocols: Abstract: Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin

Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin

By: Jeanne M. Willard³, Dennis A. Lee³, Mitchell M. Holland³

Abstract

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A wide range of biological samples are encountered in the field of forensic science, including blood, soft tissue, semen, urine, saliva, teeth, and bone. Forensic samples are routinely found as stains on various substrates, including cotton, denim, carpet, wallboard, wood, envelopes, and cigaret butts. Prior to collection, these samples have often been exposed to severe environmental conditions, such as varying degrees of temperature and humidity, microbial and chemical contaminants, and exposure to soils and other natural substances, such as salt water.

Affiliation(s): (3) The Armed Forces DNA Identification Laboratory, Office of the Armed Forces Medical Examiner, The Armed Forces Institute of Pathology, Rockville, MD

Book Title: Forensic DNA Profiling Protocols

Series: Methods in Molecular Biology | Volume: 98 | Pub. Date: Jan-22-1998 | Page Range: 9-18 | DOI: 10.1385/0-89603-443-7:9

Subject: Genetics/Genomics

References

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