SpringerProtocols: Abstract: Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis

Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis

By: Gregers J. Gram², John-Erik Stig Hansen²

Abstract

Full Text

Download PDF (665K)

The envelope glycoprotein gp120 of the human immunodeficiency virus (HIV), the causative agent of AIDS, contains approx 24 potential sites for N-glycosylation (Asn-X-Ser/Thr, X≠Pro) (1), all of which are utilized and constitute about 50% of the molecular mass (2). Binding of gp120 to the CD4 molecule is an initial step in viral infection of cells bearing the CD4 molecule on the surface, and fusion of HIV-infected cells with uninfected CD4-positive cells (3).

Affiliation(s): (2) Department of Infectious Diseases, University of Copenhagen, Hvidovere Hospital, Copenhagen, Denmark

Book Title: Lectin Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 9 | Pub. Date: Sep-23-1997 | Page Range: 167-174 | DOI: 10.1385/0-89603-396-1:167

Subject: Biochemistry

References

Download References

1.	Kornfeld, R. and Komfeld, S. (1985) Assembly of asparagine-linked oligosaccharides. Ann. Rev. Biochem. 54, 631–664.

2.	Leonard, C. K., Spellman, M. W., Riddle, L., Harris, R. J., Thomas, J. N., and Gregory, T. J. (1990) Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells J Biol Chem 265, 10,373–10,382

3.	Dalgleish, A. G, Beverley, P C L, Clapham, P R, Crawford, D H, Greaves, M F, and Weiss, R A (1984) The CD4(T4) antigen is an essential component of the receptor for the AIDS retrovirus Nature 312, 763–767

4.	Mizuochi, T, Spellmann, M V, Larkin, M., Soloman, J, Basa, L J, and Felzl, T. (1988) Carbohydrate structures of the human-immunodeficiency-virus (HIV) recombinant envelope glycoprotein gp120 produced in Chinese hamster ovary cells Biochem J 263, 599–603.

5.	Mizuochi, T, Matthews, T J, Kato, M, Hamako, J, Titani, K, Solomon, J, and Feizi, T (1990) Diversity of oligosaccharide structures on the envelope glycoprotein gp120 of human immunodeficiency virus 1 from the lymphoblastoid cell line H9 Presence of complex-type oligosaccharides with bisecting N-acetylglucosamine residues. J Biol Chem 265,8519–8524

6.	Geyer, H, Holsbach. C, Hunsmann, G., and Scheider, J. (1988) Carbohydrate of human immunodeficiency virus J Biol. Chem 263, 11,760–11,767

7.	Mathews, T J, Weinhold, K J, Lyerly, H K, Langlois, A J, Wigzell, H, and Bolognesi, D P (1987) Interaction between the human T-cell lymphotropic virus type IIIB envelope glycoprotein gp120 and the surface antigen CD4 Role of carbohydrate in binding and cell fusion Proc Natl Acad Sci USA 84, 5424–5428

8.	Fenouillet, E, Clerget-Raslain, B., Gluckmann, J C, Guetard, D., Montagnier, L, and Bahraoul, E (1989) Role of N-linked glycans in the interaction between the envelope glycoprotein of human immunodeficiency virus and its CD4 cellular receptor J Exp med 169, 807–822

9.	Gruters, R A, Neefjes, J J, Tersmette, M, de Goede, R E Y., Tulp, A, Huismann, H G, Miedema, F, and Ploegh, H L. (1987) Interference with HIV-induced syncytium formation and viral infectivity by inhibitors of trimming glycosidases. Nature 330, 74–77.

10.	Lifson, J., Coutre, S, Huang, E, and Englemann, E (1986) Role of envelope glycoprotein carbohydrate in human immunodeficiency virus (HIV) infectivity and virus-induced cell fusion J Exp Med 164,2101–2106

11.	Osawa, T. and Tsuji, T (1987) Fractionation and structural assessment of oligosaccharides and glycopeptides by use of immobilized lectins Ann Rev Biochem 56,21–42

Comments (Loading...)

Post comment/View all comments