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Useful Tools
Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports
Abstract
There are different strategies for the construction of soluble and solid phasebound chemrcal peptide libraries. These libraries have been used for the detection of epitopes as well as for the identification of peptides that act as antagonists of medically relevant proteins. We have prepared different types of cellulose-bound peptide libraries by spot synthesis (1), which is a powerful tool for the simultaneous and positionally addressable synthesis of thousands of peptrdes or peptide mixtures bound to continuous cellulose membrane supports. Presently up to 8000 different spots (peptides or peptide mixtures) can be automatically synthesized on a 20 *x 30-cm cellulose membrane and screened for ligand binding within l–2 wk.
Affiliation(s): (2) Institut für Medizinische Immunologie, Universitäatsklinikum Charité, Humboldt-Universität zu Berlin, Germany
Series: Methods in Molecular Biology  |  Volume: 87  |  Pub. Date: Dec-23-1997  |  Page Range: 25-39  |  DOI: 10.1385/0-89603-392-9:25
Subject:  Protein Science
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