SpringerProtocols: Abstract: Synthesis of a One-Bead One-Compound Combinatorial Peptide Library

Synthesis of a One-Bead One-Compound Combinatorial Peptide Library

By: Kit S. Lam², Michal Lebl²

Abstract

Full Text

Download PDF (344K)

The four general methods to generate and screen a huge combinatorial peptide library (>10⁷ peptides) are: biological libraries such as filamentous phage (1), plasmid (2), or polysome (3) libraries; the “one-bead one-compound” synthetic combinatonal library method or the “Selectlde process” (4–6); synthetic peptide library methods that require deconvolution, such as an iterative approach (7,8), positional scanning (9); orthogonal partition approach (10), or recurse deconvolution (11); and synthetic library using affinity column selection method (12,13).

Affiliation(s): (2) Arizona Cancer Center and Department of Internal Medicine, College of Medicine, Tucson, AZ

Book Title: Combinatorial Peptide Library Protocols

Series: Methods in Molecular Biology | Volume: 87 | Pub. Date: Dec-23-1997 | Page Range: 1-6 | DOI: 10.1385/0-89603-392-9:1

Subject: Protein Science

References

Download References

1.	Scott J K. and Smith G.P. (1990) Searching for peptide ligands with an epitope library. Science 249, 386–390.

2.	Schatz P. (1993) Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme A 13 residue consensus peptide specifies biotinylation in Escherichia cob Biotechnology 11, 1138–1143.

3.	Kawasaki G. (1991) Cell-free synthesis and isolation of novel genes and polypeptides. PCT International Patent Application W09l/05058.

4.	Lam K. S., Salmon S. E., Hersh E M., Hruby V. J., Kazmierski W. M., and Knapp R. J. (1991) One-bead, one-peptide: a new type of synthetic peptide library for identifying bgand-binding activity. Nature 354, 82–84

5.	Lebl M., Krchnak V., Sepetov N F., Seligmann B., Strop P., Felder S and Lam K S (1995) One-bead-one structure combinatorial libraries. Bzopolymers 37, 177–198.

6.	Lam K S., Lebl M., and Krchnak V. (1997) The “ one-bead-one-compound” combinatorial library method. Chem. Rev. 97, 411–448

7.	Geysen H. M., Rodda S J., and Mason T J. (1986) A priori delmeation of a peptide which mimics a discontmuous antigenic determinant. Mol. Immunol. 23, 709–715.

8.	Houghten R. A., et al. (1991) Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery. Nature 354, 84–86

9.	Dooley C. T and Houghten R A (1993) The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands Life Sci. 56, 1509–1517

10.	Deprez B., Willard X., Bourel L., Coste H., Hyafil F., and Tartar A (1995) Orthogonal combinatorial chemical libraries J Am. Chem. Sot. 117, 5405–5408

11.	Erb E., Janda K., and Brenner S. (1994) Recenstve deconvolution of combinatorial chemtcal libraries Proc.Nutl Acad.Scz USA 91, 11,422–11,425

12.	Zuckermann R. N., Kerr J. M., Slam M A., Banvtlle S. C., and Santa D V (1992) Identification of highest-affinity ligands by affinity selection from equimolar peptide mixtures generated by robotic synthesis. Proc Natl. Acad. Scz. USA 89, 4505–4509.

13.	Songyang Z., Carraway K L., Eck M. J., Harrtson S C., Feldman R. A., Mohammadi M., Schlessinger J., Hubbard S. R., Smith D P., Eng C., Lorenzo M. J., Ponder B. A J., Mayer B J., and Cantley L. C (1995) Catalytic specificity of protein-tyrosme kmases 1s crmcal for selective stgnalling Nature 373, 536–539

14.	Furka A., Sebestyen F., Asgedom M., and Dtbo G (1991) General method for rapid synthesis of multicomponent peptide mixtures. Int J Peptzde Protein Res 37, 487–493.

15.	Lam K. S and Lebl M (1994) Selectide technology—bead binding screening Methods 6 372–380

16.	Lebl M., Krchnak V., Salmon S E., and Lam K. S (1994) Screening of completely random one-bead-one-peptide libraries for activmes in solution Methods 6 381–387.

17.	Kaiser E., Colescott R. L., Bossinger C D., and Cook P. I. (1970) Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides Anal. Blochem. 34, 595–602.

18.	King D. S., Fields C G., and Fields G. B. (1990) A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis Int. J. Peptlde Protein Res. 36, 255–266.

Comments (Loading...)

Post comment/View all comments