SpringerProtocols: Abstract: Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs

Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs

By: Yue Zhang², Michael A. Frohman²

Abstract

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Most attempts to identify and isolate a novel cDNA result in the acquisition of clones that represent only a part of the mRNA’s complete sequence (Fig. 1). The approach described here to clone the missing sequence (cDNA ends) employs polymerase chain reaction (PCR). Since the initial reports of rapid amplification of cDNA ends (RACE) (1) or related techniques (2,3), many labs have developed significant improvements on the basic approach (4–18). The most recent hybrid version of the relatively simple classic RACE is described here, as well as a more powerful, but technically more challenging “new RACE” protocol, which is adapted from the work of a number of laboratories (19–26). Commercial RACE kits are available from Bethesda Research Laboratories (Gaithersburg, MD) (11) and Clontech (Palo Alto, CA) that are convenient, but not as powerful as the most recent versions of classic and new RACE.

Affiliation(s): (2) Department of Pharmacology, School of Medicine, SUNY at Stony Brook, NY

Book Title: cDNA Library Protocols

Series: Methods in Molecular Biology | Volume: 69 | Pub. Date: Oct-01-1996 | Page Range: 61-87 | DOI: 10.1385/0-89603-383-X:61

Subject: Genetics/Genomics

References

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