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Isolation of Messenger RNA from Plant Tissues
By: Alison Dunn2
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The starting material for any representative plant cDNA library is a supply of good-quality messenger RNA from the plant tissue
of choice. Extraction of RNA can be made from several grams of tissue or as little as 50 mg. However, large samples are generally
more representative of the genes expressed in a population of plants in response to environmental cues or at a defined stage
of development. Therefore, large-scale extraction of RNA is the method of choice for preparations to be used for cDNA libraries.
Affiliation(s): (2) Department of Biochemistry and Genetics, The Medical School, University of Newcastle, Newcastle upon Tyne, UK
Book Title: cDNA Library Protocols
Series: Methods in Molecular Biology | Volume: 69 | Pub. Date: Oct-01-1996 | Page Range: 33-38 | DOI: 10.1385/0-89603-383-X:33
Subject: Genetics/Genomics
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