PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
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Helicobacter pylori is a curved Gram-negative bacillus that infects the human gastric mucosa. Most methods used to diagnose H. pylori require the collection of gastric samples by biopsy during gastroduodenoscopy. A positive culture or visualization of the
organism by histologic examination of biopsy material can give a definitive diagnosis (1). To make a diagnosis without gastroduodenoscopy, indirect tests, such as ELISA or urea breath tests, must be relied on (2). The polymerase chain reaction (PCR) can be used to make a highly specific diagnosis from gastric juice aspirates or environmental
samples.
Affiliation(s): (3) Department of Internal Medicine, St. Louis University School of Medicine, St. Louis, MO
(4) Department of Internal Medicine, Texas A&M University College of Medicine, Central Texas Veterans Health Care System, Temple, TX
(4) Department of Internal Medicine, Texas A&M University College of Medicine, Central Texas Veterans Health Care System, Temple, TX
Book Title: Helicobacter pylori Protocols
Series: Methods in Molecular Medicine | Volume: 8 | Pub. Date: Mar-24-1997 | Page Range: 37-40 | DOI: 10.1385/0-89603-381-3:37
Subject: Microbiology
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