SpringerProtocols: Abstract: The Use of Cell and Organ Culture for the Study of Secreted Mucins

The Use of Cell and Organ Culture for the Study of Secreted Mucins

By: Anthony P. Corfield², Adil Aslam³, Stephen Wood², Baldev Singh², Christos Paraskeva²

Abstract

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Mucins are major differentiated products of mucosal cells throughout the body and are thus important markers of normal and disease development. The use of human colonic cell lines as a model of the adenoma-carcinoma sequence is of particular interest because it allows the changes in expression of mucins to be studied during the development and progression of disease (1,2). Recently, the importance of proliferation, differentiation, and apoptosis has attracted attention to the use of culture systems for the study of cell behavior in normal and disease processes (3,4). In the same way, tissue obtained from patients at surgery or as biopsies can be placed in short-term primary or organ culture to study similar changes in disease (5,6).

Affiliation(s): (2) Bristol Royal Infirmary, Department of Medicine Labs, University of Bristol, Bristol, UK
(3) Department of Medicine Labs, Bristol Royal Infirmary, University of Bristol, Bristol, UK

Book Title: Glycoanalysis Protocols

Series: Methods in Molecular Biology | Volume: 76 | Pub. Date: Feb-27-1998 | Page Range: 145-159 | DOI: 10.1385/0-89603-355-4:145

Subject: Biochemistry

References

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