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Analysis of N- and O-Glycans by Pyridylamination
Abstract
The pyridylamination method was originally described in 1978 as a means of analyzing glycan structures with high-sensitivity (1). Subsequently, the method has been applied to structure analyses of glycans including glycosidase digestion (2), 2D-mapping by various kinds of high-performance liquid chromatography (HPLC) (3), partial acetolysis (4), Smith degradation (5), methylation analysis (1), nuclear magnetic resonance (6), mass spectrometry (7), and lectin-affinity chromatography (8).
Affiliation(s): (2) Department of Chemistry, Osaka University College of Science, Osaka, Japan
Series: Methods in Molecular Biology  |  Volume: 76  |  Pub. Date: Feb-27-1998  |  Page Range: 101-113  |  DOI: 10.1385/0-89603-355-4:101
Subject:  Biochemistry
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