SpringerProtocols: Abstract: Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

By: Gloria Juan², Zbigniew Darzynkiewicz²

Abstract

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Progression of cells through successive phases and checkpoints of the cell cycle is maintained by sequential phosphorylation of different sets of nuclear and cytoplasmic proteins by cyclin-dependent kinases (CDKs) (1–12). By activating their partner CDKs and targeting them to the respective protein substrates cyclins play a key regulatory role in this process. Cyclins Bl, A, E, and D are expressed discontinuously during the cycle. The synthesis and degradation of these cyclins occurs at well-defined time points of the cell cycle (Table 1).

Table 1 Cyclins and Their Partner CDKs During the Cell Cycle

Cyclin	Primary CDK partner(s)	Presumed role in cell cycle	Peak of expression	Localization
D type	CDK4 and CDK6	pRB phosphorylation, commitment to S phase	Early in G1	Nucleus
E	CDK2	Initiation of S	G1/ S transition	Nucleus
A	CDK2 and CDC2	S and G2 traverse	During G2M	Nucleus
Bl	CDC2	G2 traverse entrance to M	Late G2/M	Cytoplasm/Nucleus^a

^aCyclin B1 is localized in cytoplasm during G2 and undergoes translocation to nucleus during prophase

Affiliation(s): (2) The Cancer Research Institute, New York Medical College, Elmsford, NY

Book Title: Flow Cytometry Protocols

Series: Methods in Molecular Biology | Volume: 91 | Pub. Date: Nov-26-1997 | Page Range: 67-75 | DOI: 10.1385/0-89603-354-6:67

Subject: Cell Biology

References

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